产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
乳腺癌1号基因抗体[17F8]-ChIP级
目录 :
GTX70111
规格 :
100.00μg
价格 :
299美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
17F8
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
GeneTex 产品网页
文章摘录数: 14
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 6a
Urban V, Dobrovolna J, Huhn D, Fryzelkova J, Bartek J, Janscak P. RECQ5 helicase promotes resolution of conflicts between replication and transcription in human cells. J Cell Biol. 2016;214:401-15 pubmed 出版商
Buisson R, Masson J. PALB2 self-interaction controls homologous recombination. Nucleic Acids Res. 2012;40:10312-23 pubmed 出版商
Bosviel R, Dumollard E, Dechelotte P, Bignon Y, Bernard Gallon D. Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?. OMICS. 2012;16:235-44 pubmed 出版商
Vardi A, Bosviel R, Rabiau N, Adjakly M, Satih S, Dechelotte P, et al. Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells. In Vivo. 2010;24:393-400 pubmed
Bernard Gallon D, Dechelotte P, Le Corre L, Chalabi N, Vissac Sabatier C, Bignon Y. Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia. Anticancer Res. 2004;24:321-4 pubmed
Ongusaha P, Ouchi T, Kim K, Nytko E, Kwak J, Duda R, et al. BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest. Oncogene. 2003;22:3749-58 pubmed
Bernard Gallon D, Dechelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, et al. BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite. Breast Cancer Res. 2001;3:61-5 pubmed
Lu M, Arrick B. Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms. Oncogene. 2000;19:6351-60 pubmed
de Toledo S, Azzam E, Dahlberg W, Gooding T, Little J. ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation. Oncogene. 2000;19:6185-93 pubmed
Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, et al. Tumor suppressor p53 is required to modulate BRCA1 expression. Mol Cell Biol. 2000;20:7450-9 pubmed
Altiok S, Batt D, Altiok N, Papautsky A, Downward J, Roberts T, et al. Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells. J Biol Chem. 1999;274:32274-8 pubmed
Bernard Gallon D, Dechelotte P, Rio P, Bignon Y. Expression of human BRCA1 and BRCA2 proteins in lung from a fetus at 19 weeks' gestation. Int J Cancer. 1999;82:771-3 pubmed
Yoshikawa K, Honda K, Inamoto T, Shinohara H, Yamauchi A, Suga K, et al. Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases. Clin Cancer Res. 1999;5:1249-61 pubmed
Bernard Gallon D, Dechelotte P, Rio P, Bignon Y. BRCA1 and BRCA2 proteins are expressed in milk fat globules. Int J Cancer. 1999;81:839-43 pubmed
产品信息
目录号 :
GTX70111
产品名称 :
乳腺癌1号基因抗体[17F8]-ChIP级
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
一抗
规格 :
100.00μg
标价 :
299美元
Pubmed号列表 :
12802282,10542266,10417779,22941656,11003642,10328242,16260778,10389907,20668305,11175350,15015615,11175332,22339411,11250747,15208681
克隆 :
17F8
抗体亚型 :
IgG1
产品描述 :
Mouse monoclonal [17F8] to BRCA1
交叉活性 :
Hu, Ms
背景 :
BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
免疫原 :
Protein fragment expressed in E. coli corresponding to amino acids 762-1315.
特异性 :
This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.
缓冲液 :
磷酸盐缓冲液, pH7.2含无防腐剂
用途 :
酶联免疫吸附测定, 免疫细胞化学/IF, 免疫组化, 免疫组化-P, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
浓度 :
1.81毫克/毫升
一般参考 :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
图像Legend_4 :
Sample (30 ug of whole cell lysate) A: 293T B: HeLa 5% SDS PAGE GTX70111 diluted at 1:500
图像Legend_5 :
BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500
更多信息或购买 :
GeneTex 产品网页
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
http://www.genetex.com
886-3-6208988
公司总部: 美国