产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
乳腺癌1号基因抗体[6B4]-ChIP级
目录 :
GTX70115
规格 :
100.00μg
价格 :
299美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
6B4
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 染色质免疫沉淀
更多信息或购买 :
GeneTex 产品网页
文章摘录数: 4
出版应用/物种/样品/稀释参考文献
  • 染色质免疫沉淀 ; 人类; 图 7
Morales J, Richard P, Patidar P, Motea E, Dang T, Manley J, et al. XRN2 Links Transcription Termination to DNA Damage and Replication Stress. PLoS Genet. 2016;12:e1006107 pubmed 出版商
Lajud S, Nagda D, Yamashita T, Zheng J, Tanaka N, Abuzeid W, et al. Dual disruption of DNA repair and telomere maintenance for the treatment of head and neck cancer. Clin Cancer Res. 2014;20:6465-78 pubmed 出版商
Bernard Gallon D, Dechelotte P, Le Corre L, Chalabi N, Vissac Sabatier C, Bignon Y. Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia. Anticancer Res. 2004;24:321-4 pubmed
Li S, Ting N, Zheng L, Chen P, Ziv Y, Shiloh Y, et al. Functional link of BRCA1 and ataxia telangiectasia gene product in DNA damage response. Nature. 2000;406:210-5 pubmed
产品信息
目录号 :
GTX70115
产品名称 :
乳腺癌1号基因抗体[6B4]-ChIP级
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
一抗
规格 :
100.00μg
标价 :
299美元
Pubmed号列表 :
10910365,15015615,25324139
克隆 :
6B4
抗体亚型 :
IgG1
产品描述 :
Mouse monoclonal [6B4] to BRCA1
交叉活性 :
Hu, Ms
背景 :
BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
免疫原 :
BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
特异性 :
This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
缓冲液 :
PBS
用途 :
免疫细胞化学/IF, 免疫组化, 免疫沉淀, 免疫印迹, 染色质免疫沉淀法
浓度 :
2.75毫克/毫升
一般参考 :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
图像Legend_5 :
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
图像Legend_6 :
BRCA1 antibody 6B4 (GTX70115) was used for Western blot assay at 1:1000 antibody dilution. Western blot assay was performed using MCF7 (human) and MEF(mouse embryonic fibroblast) cell lysate separated in a 5-15% gradient gel. Observed molecular weight of BRCA1: 220 KD. Predicted molecular weight of BRCA1: 220 KD.
图像Legend_7 :
BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70115) at a dilution of 1:500.
更多信息或购买 :
GeneTex 产品网页
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
www.genetex.com
886-3-6208988
公司总部: 美国