GeneTex

抗体

p53抗体[Pab1801]

GTX70216

100.00μg

299美元

单克隆

小鼠

nonconjugated

Pab1801

免疫印迹, 酶联免疫吸附测定, 免疫沉淀, 流式细胞仪, 放射免疫分析法, 免疫组化-石蜡切片, 免疫组化-冰冻切片

GTX70216

p53抗体[Pab1801]

小鼠

单克隆

一抗

100.00μg

299美元

20501806

Pab1801

IgG1

小鼠单克隆抗体人类p53

Hu

PAb1801 specifically recognizes human p53 protein, a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. p53, a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate that an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15 is a mutation which reduces the ability of p53 to arrest cell growth.

凝胶纯化融合蛋白含编码地区人的p53在大肠杆菌中表达

磷酸盐缓冲液, pH值7.4, 含无防腐剂

ELISA, FACS, IHC-Fr, IHC-P, IP, RIA, WB, ChIP assay

1毫克/毫升

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (GTX70216). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by GTX70216 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX225857-01) was used in Western blot.

The image shows a Western blot for GTX70216 , testing the threshold detection of the p53 antibody. The lanes are as follows:. Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane