产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
Rad51抗体[14B4]
目录 :
GTX70230
规格 :
100.00μg
价格 :
299美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
14B4
反应物种 :
人类, 小鼠,
应用 :
免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 35
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:500; 图 4a
Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200; 图 3
Zhang H, Liu H, Chen Y, Yang X, Wang P, Liu T, et al. A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice. Nat Commun. 2016;7:10201 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200; 图 3B
Uringa E, Baldeyron C, Odijk H, Wassenaar E, van Cappellen W, Maas A, et al. A mRad51-GFP antimorphic allele affects homologous recombination and DNA damage sensitivity. DNA Repair (Amst). 2015;25:27-40 pubmed 出版商
Perl A, O Connor C, Fa P, Mayca Pozo F, Zhang J, Zhang Y, et al. Protein phosphatase 2A controls ongoing DNA replication by binding to and regulating cell division cycle 45 (CDC45). J Biol Chem. 2019;294:17043-17059 pubmed 出版商
Heijink A, Everts M, Honeywell M, Richards R, Kok Y, de Vries E, et al. Modeling of Cisplatin-Induced Signaling Dynamics in Triple-Negative Breast Cancer Cells Reveals Mediators of Sensitivity. Cell Rep. 2019;28:2345-2357.e5 pubmed 出版商
Heijink A, Talens F, Jae L, van Gijn S, Fehrmann R, Brummelkamp T, et al. BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity. Nat Commun. 2019;10:100 pubmed 出版商
van Gijn S, Wierenga E, van den Tempel N, Kok Y, Heijink A, Spierings D, et al. TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells. Oncogene. 2019;38:852-867 pubmed 出版商
Mansour W, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube Magg C, et al. Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Sci Rep. 2018;8:3947 pubmed 出版商
Browning C, Wise J. Prolonged exposure to particulate chromate inhibits RAD51 nuclear import mediator proteins. Toxicol Appl Pharmacol. 2017;331:101-107 pubmed 出版商
Jiang Y, Verbiest T, Devery A, Bokobza S, Weber A, Leszczynska K, et al. Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality. Int J Radiat Oncol Biol Phys. 2016;95:772-81 pubmed 出版商
Zhu J, Chen H, Guo X, Qiu X, Hu C, Chamberlin A, et al. Synthesis, molecular modeling, and biological evaluation of novel RAD51 inhibitors. Eur J Med Chem. 2015;96:196-208 pubmed 出版商
Waddell N, Pajic M, Patch A, Chang D, Kassahn K, Bailey P, et al. Whole genomes redefine the mutational landscape of pancreatic cancer. Nature. 2015;518:495-501 pubmed 出版商
White M, Kaminski R, Khalili K, Wollebo H. Rad51 activates polyomavirus JC early transcription. PLoS ONE. 2014;9:e110122 pubmed 出版商
Pefani D, Latusek R, Pires I, Grawenda A, Yee K, Hamilton G, et al. RASSF1A-LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nat Cell Biol. 2014;16:962-71, 1-8 pubmed 出版商
Karnak D, Engelke C, Parsels L, Kausar T, Wei D, Robertson J, et al. Combined inhibition of Wee1 and PARP1/2 for radiosensitization in pancreatic cancer. Clin Cancer Res. 2014;20:5085-96 pubmed 出版商
Naipal K, Verkaik N, Ameziane N, van Deurzen C, Ter Brugge P, Meijers M, et al. Functional ex vivo assay to select homologous recombination-deficient breast tumors for PARP inhibitor treatment. Clin Cancer Res. 2014;20:4816-26 pubmed 出版商
Kaminski R, Wollebo H, Datta P, White M, Amini S, Khalili K. Interplay of Rad51 with NF-?B pathway stimulates expression of HIV-1. PLoS ONE. 2014;9:e98304 pubmed 出版商
Pfäffle H, Wang M, Gheorghiu L, Ferraiolo N, Greninger P, Borgmann K, et al. EGFR-activating mutations correlate with a Fanconi anemia-like cellular phenotype that includes PARP inhibitor sensitivity. Cancer Res. 2013;73:6254-63 pubmed 出版商
Engelke C, Parsels L, Qian Y, Zhang Q, Karnak D, Robertson J, et al. Sensitization of pancreatic cancer to chemoradiation by the Chk1 inhibitor MK8776. Clin Cancer Res. 2013;19:4412-21 pubmed 出版商
Wei D, Parsels L, Karnak D, Davis M, Parsels J, Marsh A, et al. Inhibition of protein phosphatase 2A radiosensitizes pancreatic cancers by modulating CDC25C/CDK1 and homologous recombination repair. Clin Cancer Res. 2013;19:4422-32 pubmed 出版商
Zhu J, Zhou L, Wu G, Konig H, Lin X, Li G, et al. A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia. EMBO Mol Med. 2013;5:353-65 pubmed 出版商
Oplustilova L, Wolanin K, Mistrik M, Korinkova G, Simkova D, Bouchal J, et al. Evaluation of candidate biomarkers to predict cancer cell sensitivity or resistance to PARP-1 inhibitor treatment. Cell Cycle. 2012;11:3837-50 pubmed 出版商
Borst G, McLaughlin M, Kyula J, Neijenhuis S, Khan A, Good J, et al. Targeted radiosensitization by the Chk1 inhibitor SAR-020106. Int J Radiat Oncol Biol Phys. 2013;85:1110-8 pubmed 出版商
Sharma S, Hicks J, Chute C, Brennan J, Ahn J, Glover T, et al. REV1 and polymerase ? facilitate homologous recombination repair. Nucleic Acids Res. 2012;40:682-91 pubmed 出版商
Lee M, Daniels M, Garnett M, Venkitaraman A. A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor. Oncogene. 2011;30:3360-9 pubmed 出版商
Klammer H, KADHIM M, Iliakis G. Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells. Cancer Res. 2010;70:8498-506 pubmed 出版商
Ammazzalorso F, Pirzio L, Bignami M, Franchitto A, Pichierri P. ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery. EMBO J. 2010;29:3156-69 pubmed 出版商
Graeser M, McCarthy A, Lord C, Savage K, Hills M, Salter J, et al. A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer. Clin Cancer Res. 2010;16:6159-68 pubmed 出版商
Bowen C, Gelmann E. NKX3.1 activates cellular response to DNA damage. Cancer Res. 2010;70:3089-97 pubmed 出版商
Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res. 2010;38:e129 pubmed 出版商
Dungey F, Caldecott K, Chalmers A. Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90. Mol Cancer Ther. 2009;8:2243-54 pubmed 出版商
Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D. DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression. Cell Death Differ. 2009;16:795-806 pubmed 出版商
Warren M, Lord C, Masabanda J, Griffin D, Ashworth A. Phenotypic effects of heterozygosity for a BRCA2 mutation. Hum Mol Genet. 2003;12:2645-56 pubmed
Kao G, McKenna W, Guenther M, Muschel R, Lazar M, Yen T. Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response. J Cell Biol. 2003;160:1017-27 pubmed
Masson J, Davies A, Hajibagheri N, Van Dyck E, Benson F, Stasiak A, et al. The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51. EMBO J. 1999;18:6552-60 pubmed
产品信息
目录号 :
GTX70230
产品名称 :
Rad51抗体[14B4]
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
一抗
规格 :
100.00μg
标价 :
299美元
Pubmed号列表 :
22983061,12928478,10562567,21926160,20802463,19229246,12668657,20802015,20861183,20385602,20395202,19671736,21399666,23341130,23780887,23804422,23966292,22981708,24847939,24963051,25117293,25218637,25310191,25463395,25719666,25874343
克隆 :
14B4
抗体亚型 :
IgG2b
产品描述 :
小鼠单克隆抗体人类Rad51
交叉活性 :
Hu, Ms, Chk
背景 :
Rad51, a 37 kDa protein, is the human homologue of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).
免疫原 :
全长(氨基酸1-338)Rad51在大肠杆菌中表达
缓冲液 :
磷酸盐缓冲液, pH值7.4, 含无防腐剂
用途 :
免疫细胞化学/IF, 免疫组化-P, 免疫沉淀, 免疫印迹
浓度 :
1.27毫克/毫升
一般参考 :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
图像Legend_4 :
Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51. Whole cell extract was used as input.
图像Legend_5 :
Rad51 antibody [14B4] detects Rad51 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at a dilution of 1:500 and developed with Trident Sharp-ECL (GTX14698).
更多信息或购买 :
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
http://www.genetex.com
886-3-6208988
公司总部: 美国