产品简要
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
HEC1抗体[9G3.23]
目录 :
GTX70268
规格 :
100.00μg
价格 :
299美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
9G3.23
反应物种 :
鹌鹑, 人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 52
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 1f
Fang Y, Kong Y, Xi J, Zhu M, Zhu T, Jiang T, et al. Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity. Oncotarget. 2016;7:79327-79341 pubmed 出版商
  • 免疫细胞化学; 人类; 图 2g
Chung H, Park J, Lee N, Kim H, Jang C. Phosphorylation of Astrin Regulates Its Kinetochore Function. J Biol Chem. 2016;291:17579-92 pubmed 出版商
  • 免疫细胞化学; 人类; 1:500; 图 3d
  • 免疫印迹; 人类; 1:1000; 图 3e
O Connor A, Maffini S, Rainey M, Kaczmarczyk A, Gaboriau D, Musacchio A, et al. Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint. Biol Open. 2015;5:11-9 pubmed 出版商
  • 免疫细胞化学; 人类
Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. 2015;290:27053-66 pubmed 出版商
  • 免疫印迹; 鹌鹑
Miller S, Johnson M, Stukenberg P. Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1). Curr Biol. 2008;18:1785-91 pubmed 出版商
Malaby H, Dumas M, Ohi R, Stumpff J. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol. 2019;218:1218-1234 pubmed 出版商
Alfonso Pérez T, Hayward D, Holder J, Gruneberg U, Barr F. MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. J Cell Biol. 2019;218:1108-1117 pubmed 出版商
Petsalaki E, Dandoulaki M, Zachos G. The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling. J Cell Biol. 2018;217:861-876 pubmed 出版商
Ferrara M, Sessa G, Fiore M, Bernard F, Asteriti I, Cundari E, et al. Small molecules targeted to the microtubule-Hec1 interaction inhibit cancer cell growth through microtubule stabilization. Oncogene. 2018;37:231-240 pubmed 出版商
Shirnekhi H, Kelley E, DeLuca J, Herman J. Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation. Mol Biol Cell. 2017;28:2035-2041 pubmed 出版商
Janczyk P, Skorupka K, Tooley J, Matson D, Kestner C, WEST T, et al. Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores. Dev Cell. 2017;41:438-449.e4 pubmed 出版商
Huis In t Veld P, Jeganathan S, Petrovic A, Singh P, John J, Krenn V, et al. Molecular basis of outer kinetochore assembly on CENP-T. elife. 2016;5: pubmed 出版商
Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova Y, et al. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Cell. 2016;167:1028-1040.e15 pubmed 出版商
Redli P, Gasic I, Meraldi P, Nigg E, Santamaria A. The Ska complex promotes Aurora B activity to ensure chromosome biorientation. J Cell Biol. 2016;215:77-93 pubmed
Zhou H, Wang T, Zheng T, Teng J, Chen J. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 2016;7:10151 pubmed 出版商
Tauchman E, Boehm F, DeLuca J. Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Commun. 2015;6:10036 pubmed 出版商
Espert A, Uluocak P, Bastos R, Mangat D, Graab P, Gruneberg U. PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. J Cell Biol. 2014;206:833-42 pubmed 出版商
Häfner J, Mayr M, Möckel M, Mayer T. Pre-anaphase chromosome oscillations are regulated by the antagonistic activities of Cdk1 and PP1 on Kif18A. Nat Commun. 2014;5:4397 pubmed 出版商
Huang L, Chang C, Lee Y, Huang J, Chuang S, Chang J, et al. Inhibition of Hec1 as a novel approach for treatment of primary liver cancer. Cancer Chemother Pharmacol. 2014;74:511-20 pubmed 出版商
Basilico F, Maffini S, Weir J, Prumbaum D, Rojas A, Zimniak T, et al. The pseudo GTPase CENP-M drives human kinetochore assembly. elife. 2014;3:e02978 pubmed 出版商
Matson D, Stukenberg P. CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status. J Cell Biol. 2014;205:541-54 pubmed 出版商
Petsalaki E, Zachos G. Chk2 prevents mitotic exit when the majority of kinetochores are unattached. J Cell Biol. 2014;205:339-56 pubmed 出版商
Huang L, Chang C, Lee Y, Chang J, Huang J, Chuang S, et al. Activity of a novel Hec1-targeted anticancer compound against breast cancer cell lines in vitro and in vivo. Mol Cancer Ther. 2014;13:1419-30 pubmed 出版商
Caldas G, Deluca K, DeLuca J. KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity. J Cell Biol. 2013;203:957-69 pubmed
Beck J, Maerki S, Posch M, Metzger T, Persaud A, Scheel H, et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol. 2013;15:430-9 pubmed 出版商
Buheitel J, Stemmann O. Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate. EMBO J. 2013;32:666-76 pubmed 出版商
Lawo S, Hasegan M, Gupta G, Pelletier L. Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nat Cell Biol. 2012;14:1148-58 pubmed 出版商
De Antoni A, Maffini S, Knapp S, Musacchio A, Santaguida S. A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B. J Cell Biol. 2012;199:269-84 pubmed 出版商
Matson D, Demirel P, Stukenberg P, Burke D. A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev. 2012;26:542-7 pubmed 出版商
Chan Y, Jeyaprakash A, Nigg E, Santamaria A. Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction. J Cell Biol. 2012;196:563-71 pubmed 出版商
Krenn V, Wehenkel A, Li X, Santaguida S, Musacchio A. Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction. J Cell Biol. 2012;196:451-67 pubmed 出版商
Serio G, Margaria V, Jensen S, Oldani A, Bartek J, Bussolino F, et al. Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores. Proc Natl Acad Sci U S A. 2011;108:17337-42 pubmed 出版商
Barnhart M, Kuich P, Stellfox M, Ward J, Bassett E, Black B, et al. HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J Cell Biol. 2011;194:229-43 pubmed 出版商
Santaguida S, Vernieri C, Villa F, Ciliberto A, Musacchio A. Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction. EMBO J. 2011;30:1508-19 pubmed 出版商
Screpanti E, De Antoni A, Alushin G, Petrovic A, Melis T, Nogales E, et al. Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. Curr Biol. 2011;21:391-8 pubmed 出版商
Tooley J, Miller S, Stukenberg P. The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement. Mol Biol Cell. 2011;22:1217-26 pubmed 出版商
Sundin L, Guimaraes G, DeLuca J. The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis. Mol Biol Cell. 2011;22:759-68 pubmed 出版商
Zanet J, Freije A, Ruiz M, Coulon V, Sanz J, Chiesa J, et al. A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication. PLoS ONE. 2010;5:e15701 pubmed 出版商
Zeng K, Bastos R, Barr F, Gruneberg U. Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2010;191:1315-32 pubmed 出版商
Petrovic A, Pasqualato S, Dube P, Krenn V, Santaguida S, Cittaro D, et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol. 2010;190:835-52 pubmed 出版商
Screpanti E, Santaguida S, Nguyen T, Silvestri R, Gussio R, Musacchio A, et al. A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds. PLoS ONE. 2010;5:e11603 pubmed 出版商
Santaguida S, Tighe A, D Alise A, Taylor S, Musacchio A. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol. 2010;190:73-87 pubmed 出版商
Wu G, Wei R, Cheng E, Ngo B, Lee W. Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1. Mol Biol Cell. 2009;20:4686-95 pubmed 出版商
Chan Y, Fava L, Uldschmid A, Schmitz M, Gerlich D, Nigg E, et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol. 2009;185:859-74 pubmed 出版商
Zhu H, Coppinger J, Jang C, Yates J, Fang G. FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle. J Cell Biol. 2008;183:835-48 pubmed 出版商
Wu G, Qiu X, Zhou L, Zhu J, Chamberlin R, Lau J, et al. Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal. Cancer Res. 2008;68:8393-9 pubmed 出版商
Barr A, Gergely F. MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics. Mol Cell Biol. 2008;28:7199-211 pubmed 出版商
Jang C, Wong J, Coppinger J, Seki A, Yates J, Fang G. DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement. J Cell Biol. 2008;181:255-67 pubmed 出版商
Wong J, Lerrigo R, Jang C, Fang G. Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain. Mol Biol Cell. 2008;19:2083-91 pubmed 出版商
Montembault E, Dutertre S, Prigent C, Giet R. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. J Cell Biol. 2007;179:601-9 pubmed
Wong J, Fang G. HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture. J Cell Biol. 2006;173:879-91 pubmed
Klein U, Nigg E, Gruneberg U. Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP. Mol Biol Cell. 2006;17:2547-58 pubmed
产品信息
目录号 :
GTX70268
产品名称 :
HEC1抗体[9G3.23]
宿主物种 :
小鼠
克隆性 :
单克隆
产品类型 :
一抗
规格 :
100.00μg
标价 :
299美元
Pubmed号列表 :
24344188,21407176,19029337,22331848,20657644,23071153,21187932,18321990,21987812,21270439,19026542,20624901,21768289,22371557,18411309,22426531,21325630,20819937,21353556,16571674,17998396,23361318,21187329,16769820,23086237,18809577,19468067,18922912,19776357,23455478,24694948,24798733,24862574,25006165,25038613,25048371,25246613
克隆 :
9G3.23
抗体亚型 :
IgG2a
产品描述 :
小鼠单克隆抗体人类HEC1
交叉活性 :
Hu, Ms, Pig, Hm, AGMK
背景 :
Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation.
免疫原 :
Human HEC1 protein consisting of amino acids 56-642.
缓冲液 :
磷酸盐缓冲液, pH值7.4, 含无防腐剂
用途 :
流式细胞仪, 免疫细胞化学/IF, 免疫组化, 免疫沉淀, 免疫印迹, 免疫印迹老化
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
图像Legend_4 :
1/1000 dilution staining ~80 kDa HEC 1 in Hela cell lysate (30mg per well) by Western blot.
更多信息或购买 :
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
http://www.genetex.com
886-3-6208988
公司总部: 美国