GeneTex

抗体

DDDDK标签抗体(HRP)

GTX77454

100.00μg

239美元

多克隆

鸡

HRP

免疫印迹, 酶联免疫吸附测定

GTX77454

DDDDK标签抗体(HRP)

鸡

多克隆

HRP

一抗

100.00μg

239美元

鸡多克隆抗体DDDDK标签

Chk

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Chickens were immunized with DDDDK peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, eggs were collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support then conjugated to HRP

50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2% BSA, 0.01% Thimerosal

酶联免疫吸附测定, 免疫印迹

1毫克/毫升

Keep as concentrated solution, aliquot and store at 4°C.