产品简要
信息索取 :
公司名称 :
GeneTex
产品类型 :
抗体
产品名称 :
DDDDK标签抗体
目录 :
GTX77457
规格 :
100.00μg
价格 :
239美元
克隆性 :
多克隆
宿主 :
兔
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
GeneTex 产品网页
文章摘录数: 1
已发表的文献中的应用方法／种属／稀释度参考文献
Hsu Y, Sheu J, Lin C, Yang D, Hsiao G, Ou G, et al. Trichostatin A and sirtinol suppressed survivin expression through AMPK and p38MAPK in HT29 colon cancer cells. Biochim Biophys Acta. 2012;1820:104-15 pubmed 出版商
图像
图像 1 :
GeneTex GTX77457 图像 1
DDDDK tag antibody detects DDDDK tag at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and transfected with ZNF365–fusion protein (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDDDK tag stained by DDDDK tag antibody (GTX77457) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 ?m.
图像 2 :
GeneTex GTX77457 图像 2
An ELISA plate is coated with 50 ?L of a DDDDK tag conjugated BSA at concentration of 10 ?g/mL. The peptide conjugated carrier protein is coated and detected with anti-DDDDK tag antibody (GTX77457) at concentration ranged from 125 to 1.95 ng/mL.
产品信息
目录号 :
GTX77457
产品名称 :
DDDDK标签抗体
宿主物种 :
兔
克隆性 :
多克隆
产品类型 :
一抗
规格 :
100.00μg
标价 :
239美元
Pubmed号列表 :
22155142
产品描述 :
兔多克隆抗体DDDDK标签
交叉活性 :
其他
背景 :
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
免疫原 :
Rabbits were immunized with xxxDDDDK-peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected and the antibodies were affinity purified using the peptide immobilized on a solid phase.
缓冲液 :
1XPBS, 20% 甘油(pH7)。 0.01% 硫柳汞被添加a防腐剂
用途 :
酶联免疫吸附测定, 免疫细胞化学/IF, 免疫印迹
浓度 :
1毫克/毫升
一般参考 :
Keep as concentrated solution, aliquot and store at 4°C.
更多信息或购买 :
GeneTex 产品网页
公司信息
GeneTex
2456 Alton Pkwy
Irvine, CA92606
support@genetex.com
www.genetex.com
886-3-6208988
公司总部: 美国