免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-冰冻切片
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Synthetic peptide - N-terminal region of the human LC3, isoform B protein
B isoform of LC3
三羟甲基氨基甲烷甘氨酸, 150mM氯化钠含0.05% 叠氮化钠
FACS, ICC/IF, IHC, IHC-Fr, WB
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles
Immunohistochemistry: LC3B Antibody [GTX82986] - LC3B staining in treated U87-MG cultured & subcutaneous tumors.
Immunohistochemistry: LC3B Antibody [GTX82986] - LC3B staining in gliobastoma multiform tissue.
Immunohistochemistry: LC3B Antibody [GTX82986] - Staining of treated U373-MG (human glioblastoma.