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p38重组兔单克隆抗体[SR43-04](ET1602-26)
HUABIO
目录: ET1602-26
domestic rabbit 单克隆 (SR43-04)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
文章摘录数: 1
Western blot analysis of p38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lane 4: Jurkat cell lysate
ICC staining of p38 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of p38 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SR43-04
至产商
MAPK14小鼠单克隆抗体[9-B9](EM40307)
HUABIO
目录: EM40307
小鼠 单克隆 (9-B9)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis of MAPK14 on different cell lysates using anti-MAPK14 antibody at 1/500 dilution. Positive control: Lane 1: A431 Lane 2: HepG2 Lane 3: Hela Lane 4: NIH/3T3 Lane 5: Jurkat Lane 6: 293T Lane 7: mouse placenta
ICC staining of MAPK14 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: 9-B9
至产商
磷酸化p38(T180)兔多克隆抗体(ER2001-51)
HUABIO
目录: ER2001-51
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
规格: 多克隆
价格:
至产商
磷酸化P38(Thr180 + Tyr182)兔多克隆抗体(ER2001-52)
HUABIO
目录: ER2001-52
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
规格: 多克隆
价格:
至产商
p38重组兔单克隆抗体[JF55-07](ET1702-65)
HUABIO
目录: ET1702-65
domestic rabbit 单克隆 (JF55-07)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis of p38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: THP-1 cell lysate Lane 3: SiHa cell lysate Lane 4: HL-60 cell lysate
ICC staining of p38 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-65, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of p38 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1702-65, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: JF55-07
至产商
MAPK14兔多克隆抗体(R1308-3)
HUABIO
目录: R1308-3
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Immunofluorescent staining of Hela cells using anti-MAPK14 rabbit polyclonal antibody.
Western blot analysis on different cell lysates using anti-MAPK14 rabbit polyclonal antibodies.
规格: 多克隆
价格:
至产商
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