表皮生长因子受体重组兔单克隆抗体[SZ40-19](ET1603-37)
HUABIO
目录: ET1603-37
domestic rabbit 单克隆 (SZ40-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 2

Western blot analysis of EGFR on different cell lysates using anti-EGFR at 1/500 dilution. Positive control: Line 1: Hela Line 2: A431 Line 3: HUVEC

ICC staining EGFR in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining EGFR in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: SZ40-19
至产商
磷酸化表皮生长因子受体(Y1068)重组兔单克隆抗体[SD2055](ET1612-30)
HUABIO
目录: ET1612-30
domestic rabbit 单克隆 (SD2055)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
文章摘录数: 1

ICC staining of Phospho-EGFR(Y1068) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-EGFR(Y1068) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-EGFR(Y1068) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SD2055
至产商
表皮生长因子受体兔多克隆抗体(0407-21)
HUABIO
目录: 0407-21
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of EGFR on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ICC staining EGFR in A172 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-21) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-21) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
规格: 多克隆
价格:
至产商
表皮生长因子受体小鼠单克隆抗体[B6-E5-D9](EM1701-22)
HUABIO
目录: EM1701-22
小鼠 单克隆 (B6-E5-D9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of EGFR on A431 using anti-EGFR antibody at 1/10,000 dilution.

ICC staining EGFR (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining EGFR (green) in SW480 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: B6-E5-D9
至产商
表皮生长因子受体小鼠单克隆抗体[7-F2-F8](EM1901-67)
HUABIO
目录: EM1901-67
小鼠 单克隆 (7-F2-F8)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of EGFR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: SKOV-3 cell lysate Lane 3: A549 cell lysate

ICC staining of EGFR in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-67, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: 7-F2-F8
至产商
表皮生长因子受体兔多克隆抗体(ER1512-6)
HUABIO
目录: ER1512-6
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of EGFR on HepG2 (1) and Hela (2) cell lysates using anti-EGFR antibody at 1/1,000 dilution.

ICC staining EGFR in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining EGFR in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 多克隆
价格:
至产商
表皮生长因子受体重组兔单克隆抗体[SP00-86](ET1604-44)
HUABIO
目录: ET1604-44
domestic rabbit 单克隆 (SP00-86)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of EGFR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human skin tissue lysate Lane 2: 293T cell lysate

ICC staining of EGFR in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1604-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of EGFR in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1604-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SP00-86
至产商
磷酸化表皮生长因子受体(Y1092)重组兔单克隆抗体[SJ0194](ET1606-44)
HUABIO
目录: ET1606-44
domestic rabbit 单克隆 (SJ0194)
反应物种: 人类, 小鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of Phospho-EGFR(Y1092) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-44, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate treated with EGF Lane 2: Untreated A431 cell lysate

ICC staining of Phospho-EGFR(Y1092) in BT-20 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-44, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-EGFR(Y1092) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SJ0194
至产商
磷酸化表皮生长因子受体(pY1173)重组兔单克隆抗体[SC57-04](ET1610-4
HUABIO
目录: ET1610-4
domestic rabbit 单克隆 (SC57-04)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片

ICC staining of Phospho-EGFR (pY1173) in untreated A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-EGFR (pY1173) in untreated A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-EGFR (pY1173) in untreated B16F1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SC57-04
至产商
磷酸化表皮生长因子受体(S695)重组兔单克隆抗体[SN07-36](ET1611-50)
HUABIO
目录: ET1611-50
domestic rabbit 单克隆 (SN07-36)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学
规格: 单克隆
价格: SN07-36
至产商
表皮生长因子受体兔多克隆抗体(R1511-18)
HUABIO
目录: R1511-18
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of EGFR on different cell lysate using anti-EGFR antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: A431

ICC staining EGFR in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. Counter stained with hematoxylin.
规格: 多克隆
价格:
至产商
表皮生长因子受体兔多克隆抗体(R1608-3)
HUABIO
目录: R1608-3
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of EGFR on A431 (1) and HepG2 (2) cell lysate using anti-EGFR antibody at 1/1,000 dilution.

ICC staining EGFR in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining EGFR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 多克隆
价格:
至产商