ESR1兔多克隆抗体(0803-1)
HUABIO
目录: 0803-1
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪

Western blot analysis on MCF-7 using anti-ESR1 polyclonal antibody
规格: 多克隆
价格:
至产商
雌激素受体α兔多克隆抗体(ER1803-83)
HUABIO
目录: ER1803-83
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of Estrogen Receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Rat ovary tissue lysate

Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluorª 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
规格: 多克隆
价格:
至产商
磷酸化雌激素受体α(S118)重组兔单克隆抗体[S
HUABIO
目录: ET1610-32
domestic rabbit 单克隆 (SC05-87)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of Phospho-Estrogen Receptor alpha(S118) on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ICC staining of Phospho-Estrogen Receptor alpha(S118) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-Estrogen Receptor alpha(S118) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SC05-87
至产商
雌激素受体α重组兔单克隆抗体[JE26-14](ET7110
HUABIO
目录: ET7110-60
domestic rabbit 单克隆 (JE26-14)
反应物种: 人类
应用: 免疫印迹

Western blot analysis of Estrogen Receptor alpha on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
规格: 单克隆
价格: JE26-14
至产商