小鼠 单克隆 (D5-B3)
反应物种: 人类
应用: 免疫印迹, 免疫组化-石蜡切片

Western blot analysis of Fibrinogen on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-11, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Fibrinogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-11) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: D5-B3
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小鼠 单克隆 (11-B3-D8)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: 11-B3-D8
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小鼠 单克隆 (1-A7)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: 1-A7
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