磷酸甘油醛脱氢酶小鼠单克隆抗体[5-E10](EM1101)
HUABIO
目录: EM1101
小鼠 单克隆 (5-E10)
反应物种: 镰刀霉, Sitophilus, 蚕属, 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫细胞化学
文章摘录数: 35

Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: PC12 Lane 4: NIH/3T3 Lane 5: MCF-7 Lane 6: Rabbit liver Lane 7:Zebrafish

ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: 5-E10
至产商
磷酸甘油醛脱氢酶兔多克隆抗体(R1210-1)
HUABIO
目录: R1210-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 5

Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: F9 cell lysate, untreated Lane 2: NCCIT cell lysate, untreated Lane 3: PC-12 cell lysate, untreated

ICC staining GAPDH in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1210-1) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

ICC staining GAPDH in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1210-1) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
规格: 多克隆
价格:
至产商
磷酸甘油醛脱氢酶重组兔单克隆抗体[SA30-01](ET1601-4)
HUABIO
目录: ET1601-4
domestic rabbit 单克隆 (SA30-01)
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 2

Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NCCIT cell lysate Lane 2: PC-12 cell lysate Lane 3: F9 cell lysate

Western blot analysis of GAPDH on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ICC staining of GAPDH in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SA30-01
至产商
磷酸甘油醛脱氢酶小鼠单克隆抗体[12D6](M1310-2)
HUABIO
目录: M1310-2
小鼠 单克隆 (12D6)
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫细胞化学
文章摘录数: 3

Western blot analysis of GAPDH on different cells lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: PC-12 cell lysate Lane 5: F9 cell lysate

ICC staining of GAPDH in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1310-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of GAPDH in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1310-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: 12D6
至产商
磷酸甘油醛脱氢酶(HRP共轭标签)重组兔单克隆抗体[JF81-04](ET1702-6
HUABIO
目录: ET1702-66
domestic rabbit 单克隆 (JF81-04)
反应物种: 人类, 小鼠, 大鼠, 鸡
共轭标签: HRP
应用: 免疫印迹
文章摘录数: 1

Western blot analysis of GAPDH(HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell lysate Lane 2: Hela cell lysate Lane 3: NIH/3T3 cell lysate
规格: 单克隆
价格: JF81-04
至产商
磷酸甘油醛脱氢酶小鼠单克隆抗体[12D7](EM1901-57)
HUABIO
目录: EM1901-57
小鼠 单克隆 (12D7)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-57, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-12 cell lysate Lane 3: F9 cell lysate Lane 4: A549 cell lysate

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GAPDH antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-GAPDH antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: 12D7
至产商
磷酸甘油醛脱氢酶兔多克隆抗体(ER1706-83)
HUABIO
目录: ER1706-83
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of GAPDH on different lysates using anti-GAPDH antibody at 1/2,000 dilution. Positive control: Lane 1: PC-12 Lane 2: L929 Lane 3: NIH/3T3 Lane 4: F9 Lane 5: Mouse liver tissue

ICC staining GAPDH in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
规格: 多克隆
价格:
至产商
磷酸甘油醛脱氢酶兔多克隆抗体(ER1901-65)
HUABIO
目录: ER1901-65
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of GAPDH on PC-12 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell lysate Lane 2: Hela cell lysate Lane 3: Mouse ovary tissue lysate Lane 4: Human placenta tissue lysate Lane 5: Rat brain tissue lysate Lane 6: NCCIT cell lysate

ICC staining of GAPDH in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1901-65) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
规格: 多克隆
价格:
至产商
磷酸甘油醛脱氢酶兔多克隆抗体(R1108-1)
HUABIO
目录: R1108-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Western blot analysis on A: NCCIT B: F9 C: PC12 D: Human kidney cell lysates using anti-GAPDH polyclonal antibody
规格: 多克隆
价格:
至产商