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GPX1小鼠单克隆抗体[A6-C0-B9](EM1701-96)
HUABIO
目录: EM1701-96
小鼠 单克隆 (A6-C0-B9)
反应物种:
人类
应用:
免疫印迹
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis of Glutathione Peroxidase 1 on THP-1 cell lysate using anti- Glutathione Peroxidase 1 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: A6-C0-B9
至产商
谷胱甘肽过氧化物酶 1小鼠单克隆抗体[C5-A10](EM1901-56)
HUABIO
目录: EM1901-56
小鼠 单克隆 (C5-A10)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
Western blot analysis of Glutathione peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: 293 cell lysate
ICC staining of Glutathione peroxidase 1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-56, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Flow cytometric analysis of Glutathione peroxidase 1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-56, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
规格: 单克隆
价格: C5-A10
至产商
谷胱甘肽过氧化物酶 1小鼠单克隆抗体[C5-F6](EM40708)
HUABIO
目录: EM40708
小鼠 单克隆 (C5-F6)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution. Positive control: Lane 1: THP-1 Lane 2: HepG2 Lane 3: 293T
ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: C5-F6
至产商
谷胱甘肽过氧化物酶 1重组兔单克隆抗体[JJ092-07](ET17
HUABIO
目录: ET1701-84
domestic rabbit 单克隆 (JJ092-07)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫沉淀
Western blot analysis of Glutathione Peroxidase 1 on different lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: THP-1
规格: 单克隆
价格: JJ092-07
至产商
谷胱甘肽过氧化物酶 1重组兔单克隆抗体[JF0944](ET1702
HUABIO
目录: ET1702-09
domestic rabbit 单克隆 (JF0944)
反应物种:
人类
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
免疫组化-石蜡切片
Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.
ICC staining Glutathione Peroxidase 1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Glutathione Peroxidase 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: JF0944
至产商
谷胱甘肽过氧化物酶 1兔多克隆抗体(R1401-12)
HUABIO
目录: R1401-12
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis of Glutathione Peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate, untreated Lane 2: Human liver tissue lysate, untreated
ICC staining Glutathione Peroxidase 1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1401-12) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glutathione Peroxidase 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (R1401-12) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
规格: 多克隆
价格:
至产商
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