组蛋白H3小鼠单克隆抗体[3-C4](EM30605)
HUABIO
目录: EM30605
小鼠 单克隆 (3-C4)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化-石蜡切片
文章摘录数: 4

Western blot analysis of Histone H3 on different cell lysates using anti- Histone H3 antibody at 1/5000 dilution.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: 3-C4
至产商
组蛋白H3小鼠单克隆抗体[A11-D7](M1309-1)
HUABIO
目录: M1309-1
小鼠 单克隆 (A11-D7)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 2

Western blot analysis of Histone H3 on different cell lysates using anti-Histone H3 antibody at 1/2,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: F9

Immunofluorescent staining of Hela cells using anti-Histone H3 Mouse mAb.

Immunofluorescent staining of F9 cells using anti-Histone H3 Mouse mAb.
规格: 单克隆
价格: A11-D7
至产商
组蛋白H3兔多克隆抗体(R1105-1)
HUABIO
目录: R1105-1
domestic rabbit 多克隆
反应物种: 环病毒属, 人类, 小鼠, pigs
应用: 免疫印迹
文章摘录数: 2

Western blot analysis on A:HepG2 B:NIH/3T3 C:Rat liver cell lysates using anti-Histone H3 polyclonal antibody.
规格: 多克隆
价格:
至产商
乙酰组蛋白H3(K56)重组兔单克隆抗体[SU30-10](ET1608
HUABIO
目录: ET1608-9
domestic rabbit 单克隆 (SU30-10)
反应物种: 人类, 小鼠
修饰: 乙酰化
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 1

ICC staining of Histone H3(acetyl K56) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Histone H3(acetyl K56) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Histone H3(acetyl K56) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-9, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SU30-10
至产商
组蛋白H3小鼠单克隆抗体[6-A7](M1306-4)
HUABIO
目录: M1306-4
小鼠 单克隆 (6-A7)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1

Western blot analysis on Hela (1), NIH/3T3 (2), PC12(3) cell lysates using anti- Histone H3 Mouse mAb (Cat. # M1306-4).

ICC staining Histone H3 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Histone H3 mouse monoclonal antibody.
规格: 单克隆
价格: 6-A7
至产商
组蛋白H3.1兔多克隆抗体(ER1802-79)
HUABIO
目录: ER1802-79
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of Histone H3.1 on F9 cell lysate using anti-Histone H3.1 antibody at 1/1,000 dilution.

ICC staining Histone H3.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 多克隆
价格:
至产商
Mono-Methyl-Histone H3 (K18) Recombinant Rabbit Monoclonal Antibody [SA42-07] (E ...
HUABIO
目录: ET1601-14
domestic rabbit 单克隆 (SA42-07)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of Histone H3(mono methyl K18) on different cell lysates using anti-Histone H3(mono methyl K18) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2

Western blot analysis of Histone H3(mono methyl K18) on mouse testis lysates using anti-Histone H3(mono methyl K18) antibody at different dilutions. Dilution: Lane 1: 1:2000 Lane 2: 1:5000

ICC staining Histone H3(mono methyl K18) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: SA42-07
至产商
磷酸化组蛋白H3(S10)重组兔单克隆抗体[SA31-01](ET160
HUABIO
目录: ET1601-30
domestic rabbit 单克隆 (SA31-01)
反应物种: 人类, 小鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片

Western blot analysis of Phospho-Histone H3(S10) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Untreated Hela cell lysate Lane 2: Hela cell lysate treated with calyculin A Lane 3: Hela cell lysate treated with calyculin A and alkaline phosphatase

ICC staining of Phospho-Histone H3(S10) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-Histone H3(S10) in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SA31-01
至产商
Mono+Di-Methyl-Histone H3 (K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] ...
HUABIO
目录: ET1602-41
domestic rabbit 单克隆 (SR42-06)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化-石蜡切片

Western blot analysis of Histone H3(mono+di+methyl K79) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse testis tissue lysate Lane 2: CRC cell lysate

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3(mono+di+methyl K79) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Histone H3(mono+di+methyl K79) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SR42-06
至产商
Mono-Methyl-Histone H3 (K36) Recombinant Rabbit Monoclonal Antibody [SR04-20] (E ...
HUABIO
目录: ET1602-48
domestic rabbit 单克隆 (SR04-20)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学

Western blot analysis of Histone H3(mono methyl K36) on different lysates using anti-Histone H3(mono methyl K36) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
规格: 单克隆
价格: SR04-20
至产商
Mono-Methyl-Histone H3 (R2) Recombinant Rabbit Monoclonal Antibody [ST0427] (ET1 ...
HUABIO
目录: ET1609-8
domestic rabbit 单克隆 (ST0427)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学

ICC staining Histone H3(mono methyl R2) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining Histone H3(mono methyl R2) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining Histone H3(mono methyl R2) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: ST0427
至产商
Di-Methyl-Histone H3 (K9) Recombinant Rabbit Monoclonal Antibody [SN07-30] (ET16 ...
HUABIO
目录: ET1611-51
domestic rabbit 单克隆 (SN07-30)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学

ICC staining Histone H3(di methyl K9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: SN07-30
至产商
组蛋白H3重组兔单克隆抗体[JJ090-07](ET1701-64)
HUABIO
目录: ET1701-64
domestic rabbit 单克隆 (JJ090-07)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 染色质免疫沉淀 , 免疫组化-石蜡切片

Western blot analysis of Histone H3 on different lysates using anti-Histone H3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: NIH/3T3

ICC staining Histone H3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: JJ090-07
至产商
组蛋白H3重组兔单克隆抗体[JJ092-08](ET1701-81)
HUABIO
目录: ET1701-81
domestic rabbit 单克隆 (JJ092-08)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of Histone H3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: CRC cell lysate Lane 2: Hela cell lysate Lane 3: MCF-7 cell lysate Lane 4: NIH/3T3 cell lysate

ICC staining of Histone H3 in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-81, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: JJ092-08
至产商
乙酰组蛋白H3(K14)重组兔单克隆抗体[JU43-26](ET1706
HUABIO
目录: ET1706-28
domestic rabbit 单克隆 (JU43-26)
反应物种: 人类, 小鼠
修饰: 乙酰化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片

Western blot analysis of Histone H3 (acetyl K14) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1706-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate

ICC staining of Histone H3 (acetyl K14) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1706-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Histone H3 (acetyl K14) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1706-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: JU43-26
至产商
Tri-Methyl-Histone H3 (K9) Mouse Monoclonal Antibody [1-6] (M1112-3)
HUABIO
目录: M1112-3
小鼠 单克隆 (1-6 USD)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of Tri-Methyl-Histone H3 (Lys9) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1112-3, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane A: F9 cell lysate Lane B: F9+non-methyl peptide Lane C: F9+Tri-methl (lys9) peptide Lane D: PC12 cell lysate Lane E: NCCIT cell lysate

ICC staining of Tri-Methyl-Histone H3 (Lys9) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1112-3, 1/500) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Tri-Methyl-Histone H3 (Lys9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1112-3, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: 1-6美元
至产商
Mono-Methyl-Histone H3 (Lys4) Rabbit Polyclonal Antibody (R1110-1)
HUABIO
目录: R1110-1
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of Mono-mehtyl-Histone H3(Lys4) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1110-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: A: NCCIT B: purified 293T Histone C: F9 D: F9 with Histone H3 peptide-unmodifed E: F9 with Histone H3 peptide-mono methyl K4 lysates using anti-Histone H3(mono methyl K4) polyclonal antibody.

ICC staining of Mono-mehtyl-Histone H3(Lys4) in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1110-1, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Mono-mehtyl-Histone H3(Lys4) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1110-1, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 多克隆
价格:
至产商
Di-Methyl-Histone H3 (Lys4) Rabbit Polyclonal Antibody (R1110-3)
HUABIO
目录: R1110-3
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of Di-mehtyl-Histone H3(Lys4) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human liver tissue lysate, untreated Lane 2: Histone lysate, purified from 293T Lane 3: F9 cell lysate, untreated Lane 4: F9 cell lysate, treated with Histone H3 peptide-unmodifed Lane 5: F9 cell lysate, treated with Histone H3 peptide-di-methyl K4

ICC staining Di-mehtyl-Histone H3(Lys4) in Ags cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1110-3) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

ICC staining Di-mehtyl-Histone H3(Lys4) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (R1110-3) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
规格: 多克隆
价格:
至产商
乙酰组蛋白H3(K27)兔多克隆抗体(HA500046)
HUABIO
目录: HA500046
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化

Western blot analysis of Histone H3 (acetyl K27) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h

Western blot analysis of Histone H3 (acetyl K27) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with Sodium Butyrate 0.5mM for 24h

Western blot analysis of Histone H3 (acetyl K27)on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate, untreated Lane 2: NIH/3T3 cell lysate, treated with trichostatin at 500 ng/ml for 4h
规格: 100微升
价格:
至产商
乙酰组蛋白H3(K18)兔多克隆抗体(HA500047)
HUABIO
目录: HA500047
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化

Western blot analysis of Histone H3 (acetyl K18) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500047, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with Sodium Butyrate 0.5mM for 24h

Western blot analysis of Histone H3 (acetyl K18) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500047, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H3 (acetyl K18) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500047, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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