ICAM-1小鼠单克隆抗体[J0-F7](M1511-6)
HUABIO
目录: M1511-6
小鼠 单克隆 (J0-F7)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
文章摘录数: 1

Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysate

ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: J0-F7
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细胞间粘附分子1兔多克隆抗体(ER131207)
HUABIO
目录: ER131207
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of ICAM1 on different cell lysates using anti- ICAM1 antibody at 1/500 dilution. Positive control: Lane 1: Raji Lane 2: HUVEC Lane 3: K562

ICC staining of ICAM1 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
规格: 多克隆
价格:
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细胞间粘附分子1重组兔单克隆抗体[ST0487](ET1609-46)
HUABIO
目录: ET1609-46
domestic rabbit 单克隆 (ST0487)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of ICAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: HUVEC cell lysate

ICC staining of ICAM1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of ICAM1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-46, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: ST0487
至产商
细胞间粘附分子1小鼠单克隆抗体[F11-A4](M1505-7)
HUABIO
目录: M1505-7
小鼠 单克隆 (F11-A4)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis on HUVEC cell lysates using anti-ICAM1 mouse mAb.

ICC staining ICAM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: F11-A4
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