domestic rabbit 多克隆
反应物种: 人类
应用: 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类
应用: 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin B Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0807-11, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of Insulin B Chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (0807-11, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
规格: 多克隆
价格:
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小鼠 单克隆 (5E1)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
小鼠 单克隆 (A6-6)
反应物种: 人类, 小鼠, 大鼠
应用: 酶联免疫吸附测定, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 酶联免疫吸附测定, 免疫组化-石蜡切片
domestic rabbit 单克隆 (SA0410)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫细胞化学, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫细胞化学, 免疫组化-石蜡切片
Immunofluorescence staining of paraffin- embedded mouse pancreas tissue using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1601-12 at 1/200 dilution for 10 hours at 4_ and detected using Alexa Fluor¨ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Insulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-12, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-12, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SA0410
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