Smad3重组兔单克隆抗体[SY25-01](ET1607-41)
HUABIO
目录: ET1607-41
domestic rabbit 单克隆 (SY25-01)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1

Western blot analysis of Smad3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: mouse spleen tissue lysate

ICC staining of Smad3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Smad3 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SY25-01
至产商
磷酸化Smad3(S423/S425)重组兔单克隆抗体[ST0493](ET160
HUABIO
目录: ET1609-41
domestic rabbit 单克隆 (ST0493)
反应物种: 人类, 小鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

ICC staining Phospho-Smad3(S423/S425) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3(S423/S425) antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: ST0493
至产商
SMAD3兔多克隆抗体(R1510-24)
HUABIO
目录: R1510-24
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody.

Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody.

Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody.
规格: 多克隆
价格:
至产商
SMAD3兔多克隆抗体(R1510-25)
HUABIO
目录: R1510-25
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody.

Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody.

Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody.
规格: 多克隆
价格:
至产商