ERK1/2重组兔单克隆抗体[SA43-03](ET1601-29)
HUABIO
目录: ET1601-29
domestic rabbit 单克隆 (SA43-03)
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪
文章摘录数: 2

Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SW480 cell lysate Lane 3: HCT116 cell lysate Lane 4: PC-12 cell lysate

Western blot analysis of ERK1/2 on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ICC staining of ERK1/2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-29, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SA43-03
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Phospho-Erk1(pT202/pY204)+Erk2(pT185/pY187) Recombinant Rabbit Monoclonal Antibo ...
HUABIO
目录: ET1610-13
domestic rabbit 单克隆 (SC58-01)
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫组化, 免疫沉淀
文章摘录数: 2

Western blot analysis of Erk1(pT202/pY204)+Erk2(pT185/pY187) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: MCF-7 cell lysate Lane 3: SK-Br-3 cell lysate
规格: 单克隆
价格: SC58-01
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ERK1重组兔单克隆抗体[SP05-09](ET1604-32)
HUABIO
目录: ET1604-32
domestic rabbit 单克隆 (SP05-09)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
规格: 单克隆
价格: SP05-09
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ERK1兔多克隆抗体(ER131011)
HUABIO
目录: ER131011
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: SW480 Lane 3: HCT116 Lane 4: HepG2 Lane 5: PC12

ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
规格: 多克隆
价格:
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磷酸化Erk1(T202)+Erk2(T185)重组兔单克隆抗体[SZ2-4](ET
HUABIO
目录: ET1603-22
domestic rabbit 单克隆 (SZ2-4)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

ICC staining of Phospho-Erk1(T202)+Erk2(T185) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-Erk1(T202)+Erk2(T185) in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-Erk1(T202)+Erk2(T185) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SZ2-4
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ERK1重组兔单克隆抗体[SP05-09](ET1604-16)
HUABIO
目录: ET1604-16
domestic rabbit 单克隆 (SP05-09)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562

ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: SP05-09
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ERK1小鼠单克隆抗体[7-D6-E5](M1505-9)
HUABIO
目录: M1505-9
小鼠 单克隆 (7-D6-E5)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学

Western blot analysis on Hela cell lysates using anti-ERK1 mouse mAb.

ICC staining ERK1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: 7-D6-E5
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