domestic rabbit 单克隆 (SZ22-01)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, 斑马鱼
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, 斑马鱼
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
Western blot analysis of MEK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate
ICC staining of MEK1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of MEK1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-20, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SZ22-01
至产商
domestic rabbit 单克隆 (ST0490)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
Western blot analysis of Phospho-MEK1(S218/S222) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 2: 293T cell lysate
ICC staining of Phospho-MEK1(S218/S222) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-MEK1(S218/S222) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: ST0490
至产商
domestic rabbit 单克隆 (SD206-7)
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
Western blot analysis of Phospho-MEK1 (S298) on different lysates using anti-Phospho-MEK1 (S298) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431
ICC staining Phospho-MEK1 (S298) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Phospho-MEK1 (S298) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: SD206-7
至产商
domestic rabbit 单克隆 (SD2088)
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹
反应物种: 人类
修饰: 磷酸化
应用: 免疫印迹
微博分享
关注我们的微博
- 来邦网
- 英文来邦