domestic rabbit 单克隆 (SD087-05)
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹
Western blot analysis of Phospho-Raf1 (S43) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: 293T cell lysate
规格: 单克隆
价格: SD087-05
至产商
domestic rabbit 单克隆 (SD85-07)
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫组化-石蜡切片
Western blot analysis of Phospho-Raf1 (S259) on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Phospho-Raf1 (S259) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-87, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SD85-07
至产商
domestic rabbit 单克隆 (JJ086-06)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
Western blot analysis of Raf1 on HepG2 cells lysates using anti-Raf1 antibody at 1/1,000 dilution.
ICC staining Raf1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Raf1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: JJ086-06
至产商
domestic rabbit 单克隆 (JJ085-05)
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹
反应物种: 人类, 小鼠, 大鼠
修饰: 磷酸化
应用: 免疫印迹
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