domestic rabbit 单克隆 (ST48-04)
反应物种: Portunus, 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片
文章摘录数: 1
反应物种: Portunus, 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片
文章摘录数: 1
Western blot analysis of Hsp60 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: SW480 cell lysate Lane 4: PANC-1 cell lysate
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Hsp60 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Hsp60 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: ST48-04
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domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
小鼠 单克隆 (1-80 USD)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: PC12 Lane 5: F9
ICC staining HSP60 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: 1-80美元
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小鼠 单克隆 (9-49 USD)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Western blot analysis on Hela lysates using anti-HSP60 Mouse mAb (Cat. # M1007-9).
Immunofluorescent staining of Hela cells using anti-HSP60 Mouse mAb (Cat. # M1007-9).
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-HSP60 Mouse mAb (Cat. # M1007-9).
规格: 单克隆
价格: 9-49美元
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