domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 流式细胞仪
规格: 多克隆
价格:
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小鼠 单克隆 (D7-D11)
反应物种: 人类
应用: 免疫印迹
小鼠 单克隆 (4-E10)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: 4-E10
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小鼠 单克隆 (A11-A3)
反应物种: 人类
应用: 流式细胞仪, 免疫组化-石蜡切片

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-15) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-15) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Flow cytometric analysis of Transferrin was done on HepG2 cells. The cells were fixed, permeabilized and stained with Transferrin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluorª 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.
规格: 单克隆
价格: A11-A3
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小鼠 单克隆 (4-A1)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: 4-A1
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小鼠 单克隆 (E4-E6)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

ICC staining Transferrin in H22 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Transferrin monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

ICC staining Transferrin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Transferrin monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-17) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: E4-E6
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小鼠 单克隆 (C6-8)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: C6-8
至产商
小鼠 单克隆 (E6-E10)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: E6-E10
至产商
小鼠 单克隆 (6-3 USD)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
规格: 单克隆
价格: 6-3美元
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domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学