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p53小鼠单克隆抗体[7-5](EM20603)
HUABIO
目录: EM20603
小鼠 单克隆 (7-5 USD)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
文章摘录数: 1
Western blot analysis of p53 on different cell lysates using anti-p53 antibody at 1/1000 dilution. Positive control: Lane 1: 293T Lane 2: A431
ICC staining p53 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue using anti-p53 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: 7-5美元
至产商
磷酸化p53(S376)重组兔单克隆抗体[ST0440](ET1609-14)
HUABIO
目录: ET1609-14
domestic rabbit 单克隆 (ST0440)
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
文章摘录数: 1
Western blot analysis of Phospho-p53(S376) on human skin lysates using anti-Phospho-p53(S376) antibody at 1/1,000 dilution.
规格: 单克隆
价格: ST0440
至产商
p53小鼠单克隆抗体[6B2](EM1701-91)
HUABIO
目录: EM1701-91
小鼠 单克隆 (6B2)
反应物种:
人类
应用:
免疫印迹
Western blot analysis of p53 on 239T using anti-p53 antibody at 1/1,000 dilution.
规格: 单克隆
价格: 6B2
至产商
p53小鼠单克隆抗体[6B5](EM1701-92)
HUABIO
目录: EM1701-92
小鼠 单克隆 (6B5)
反应物种:
人类
应用:
免疫印迹
Western blot analysis of p53 on 239T using anti-p53 antibody at 1/500 dilution.
规格: 单克隆
价格: 6B5
至产商
p53重组兔单克隆抗体[SA39-07](ET1601-13)
HUABIO
目录: ET1601-13
domestic rabbit 单克隆 (SA39-07)
反应物种:
人类
,
斑马鱼
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis of p53 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: 293 cell lysate
Western blot analysis of p53 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of p53 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1601-13, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SA39-07
至产商
乙酰p53(K370)重组兔单克隆抗体[SR40-09](ET1602-38)
HUABIO
目录: ET1602-38
domestic rabbit 单克隆 (SR40-09)
反应物种:
人类
,
小鼠
,
大鼠
修饰: 乙酰化
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
Western blot analysis of p53(acetyl K370) on HepG2 cell lysates using anti-p53(acetyl K370) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates Lane 2: HepG2 untreated whole cell lysates
ICC staining of p53(acetyl K370) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of p53(acetyl K370) in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-38, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SR40-09
至产商
p53重组兔单克隆抗体[SY010-6](ET1605-16)
HUABIO
目录: ET1605-16
domestic rabbit 单克隆 (SY010-6)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
免疫组化-石蜡切片
Western blot analysis of p53 on 293 cells lysates using anti-p53 antibody at 1/1,000 dilution.
ICC staining p53 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p53 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: SY010-6
至产商
磷酸化p53(S392)重组兔单克隆抗体[SI17-04](ET1606-24)
HUABIO
目录: ET1606-24
domestic rabbit 单克隆 (SI17-04)
反应物种:
人类
,
小鼠
,
大鼠
修饰: 磷酸化
应用:
免疫印迹
,
免疫沉淀
,
免疫组化-石蜡切片
Western blot analysis of Phospho-p53(S392) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: F9 cell lysate Lane 3: A431 cell lysate
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Phospho-p53(S392) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Phospho-p53(S392) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SI17-04
至产商
磷酸化p53(T55)重组兔单克隆抗体[ST0439](ET1609-13)
HUABIO
目录: ET1609-13
domestic rabbit 单克隆 (ST0439)
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
Western blot analysis of Phospho-p53 (T55) on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
规格: 单克隆
价格: ST0439
至产商
乙酰p53(K382)重组兔单克隆抗体[SD0801](ET1612-71)
HUABIO
目录: ET1612-71
domestic rabbit 单克隆 (SD0801)
反应物种:
人类
修饰: 乙酰化
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis of p53 (acetyl K382) on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of p53 (acetyl K382) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of p53 (acetyl K382) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-71, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SD0801
至产商
突变体p53重组兔单克隆抗体[JB42-26](ET7107-33)
HUABIO
目录: ET7107-33
domestic rabbit 单克隆 (JB42-26)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis of Mutant p53 on 293 cell using anti-Mutant p53 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Mutant p53 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Mutant p53 antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: JB42-26
至产商
P53小鼠单克隆抗体[C8-A11](M1312-2)
HUABIO
目录: M1312-2
小鼠 单克隆 (C8-A11)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis on 293 cell lysates using anti- p53 mouse mAb.
ICC staining p53 in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti- p53 mouse monoclonal antibody.
规格: 单克隆
价格: C8-A11
至产商
P53兔多克隆抗体(R1308-7)
HUABIO
目录: R1308-7
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
Western blot analysis on 293 cell lysates using anti-p53 rabbit polyclonal antibodies.
Immunofluorescent staining of A431 cells using anti- p53 rabbit polyclonal antibody.
规格: 多克隆
价格:
至产商
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