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> HUABIO 胱氨酸-天冬氨酸蛋白酶3 抗体
胱氨酸-天冬氨酸蛋白酶3兔多克隆抗体(ER30804)
HUABIO
目录: ER30804
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
文章摘录数: 4
Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution. Positive control: Lane 1: Human lung Lane 2: Human brain Lane 3: Jurkat Lane 4: F9 Lane 5: NCCTI Lane 6: Daudi Lane 7: Raji Lane 8: MCF-7 Lane 9: Hela
ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 多克隆
价格:
至产商
胱氨酸-天冬氨酸蛋白酶3重组兔单克隆抗体[SR03-01](ET1602-39)
HUABIO
目录: ET1602-39
domestic rabbit 单克隆 (SR03-01)
反应物种:
人类
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
流式细胞仪
,
免疫组化-石蜡切片
文章摘录数: 1
Western blot analysis of Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 2: 293 cell lysate
ICC staining of Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SR03-01
至产商
活性的胱氨酸-天冬氨酸蛋白酶3重组兔单克隆抗体[SR01-02](ET1602-47)
HUABIO
目录: ET1602-47
domestic rabbit 单克隆 (SR01-02)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
文章摘录数: 1
Western blot analysis of Active Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-47, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Camptothecin (2 _M) treated Jurkat cell lysate Lane 2: Untreated Jurkat cell lysate
ICC staining of Active Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Active Caspase-3 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SR01-02
至产商
胱氨酸-天冬氨酸蛋白酶3兔多克隆抗体(ER1802-42)
HUABIO
目录: ER1802-42
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis of Caspase-3 on 293 cell lysate using anti-Caspase-3 antibody at 1/500 dilution.
ICC staining Caspase-3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Caspase-3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 多克隆
价格:
至产商
pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SZ02-08] (ET1603-26)
HUABIO
目录: ET1603-26
domestic rabbit 单克隆 (SZ02-08)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis of pro Caspase-3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of pro Caspase-3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SZ02-08
至产商
active+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SU38-04] (ET1608-64 ...
HUABIO
目录: ET1608-64
domestic rabbit 单克隆 (SU38-04)
反应物种:
人类
应用:
免疫印迹
,
免疫沉淀
,
免疫组化-石蜡切片
Western blot analysis of active+pro Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: human lung tissue lysate
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-active+pro Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-active+pro Caspase-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: SU38-04
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