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CD4兔多克隆抗体(0108-6)
HUABIO
目录: 0108-6
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
规格: 多克隆
价格:
至产商
CD4兔多克隆抗体(ER1706-80)
HUABIO
目录: ER1706-80
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis of CD4 on different tissue lysates using anti-CD4 antibody at 1/500 dilution. Positive control: Lane 1: Mouse spleen Lane 2: Mouse thymus
ICC staining CD4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 多克隆
价格:
至产商
CD4兔多克隆抗体(ER1803-84)
HUABIO
目录: ER1803-84
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
流式细胞仪
Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U937 cell lysate Lane 2: Human thymus tissue lysate
Flow cytometric analysis of CD4 was done on Hela cells. The cells were fixed, permeabilized and stained with CD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluorª 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
规格: 多克隆
价格:
至产商
CD4重组兔单克隆抗体[ST0488](ET1609-52)
HUABIO
目录: ET1609-52
domestic rabbit 单克隆 (ST0488)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U937 cell lysate Lane 2: THP-1 cell lysate Lane 3: human thymus tissue lysate
ICC staining of CD4 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of CD4 in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: ST0488
至产商
CD4重组兔单克隆抗体[JE56-36](ET7110-64)
HUABIO
目录: ET7110-64
domestic rabbit 单克隆 (JE56-36)
反应物种:
人类
应用:
免疫印迹
,
免疫组化-石蜡切片
Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: HL-60 cell lysate
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-64, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
规格: 单克隆
价格: JE56-36
至产商
CD4兔多克隆抗体(R1211-1)
HUABIO
目录: R1211-1
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Western blot analysis on K562 (1) and Daudi (2) cell lysates using anti-CD4 polyclonal antibody.
规格: 多克隆
价格:
至产商
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