CD19小鼠单克隆抗体[A3-A8](EM40308)
HUABIO
目录: EM40308
小鼠 单克隆 (A3-A8)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1

Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM40308, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysate

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40308, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of CD19 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM40308, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
规格: 单克隆
价格: A3-A8
至产商
CD19重组兔单克隆抗体[JF099-9](ET1702-74)
HUABIO
目录: ET1702-74
domestic rabbit 单克隆 (JF099-9)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪

Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: 293 cell lysate Lane 3: Jurkat cell lysate Lane 4: rat brain tissue lysate

Flow cytometric analysis of CD19 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
规格: 单克隆
价格: JF099-9
至产商
CD19重组兔单克隆抗体[JF100-06](ET1702-93)
HUABIO
目录: ET1702-93
domestic rabbit 单克隆 (JF100-06)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

Western blot analysis of CD19 on Raji cells lysates using anti-CD19 antibody at 1/1,000 dilution.

ICC staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
规格: 单克隆
价格: JF100-06
至产商
CD19兔多克隆抗体(R1107-7)
HUABIO
目录: R1107-7
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹

Western blot analysis on F9 (A) and NCCIT (B) cell lysates using anti-CD19 rabbit polyclonal antibody.
规格: 多克隆
价格:
至产商