domestic rabbit 单克隆 (SY0287)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 2
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 2
Western blot analysis of E-Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: MCF-7 cell lysate
ICC staining of E-Cadherin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of E-Cadherin in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor¨488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
规格: 单克隆
价格: SY0287
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小鼠 单克隆 (3-F9)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 1
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
Western blot analysis of E-Cadherin on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining E-Cadherin in HCT116 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-25) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
ICC staining E-Cadherin in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-25) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
规格: 多克隆
价格:
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小鼠 单克隆 (A0-G11-2)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
Western blot analysis of E-cadherin on different cell lysates using anti- E-cadherin antibody at 1/1000 dilution. Positive control: Lane 1: A431 Lane 2: SW480
ICC staining E-Cadherin in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using E-Cadherin antibody. Counter stained with hematoxylin.
规格: 单克隆
价格: A0-G11-2
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