产品简要
公司名称 :
HUABIO
产品类型 :
抗体
产品名称 :
p16INK4a小鼠单克隆抗体[A4A5](HA600026)
目录 :
HA600026
规格 :
100微升
价格 :
A4A5
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
A4A5
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
Western blot analysis of p16INK4a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600026, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: Hela cell lysate
图像 2 :
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma tissue using anti-p16INK4a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600026, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
图像 3 :
Immunohistochemical analysis of paraffin-embedded human cervical poorly differentiated adenocarcinoma tissue using anti-p16INK4a antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600026, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
产品信息
Catalog# :
HA600026
产品名称 :
p16INK4a小鼠单克隆抗体[A4A5](HA600026)
类别 :
Mouse monoclonal primary
共轭 :
未共轭
克隆性 :
单克隆
克隆编号 :
A4A5
宿主 :
小鼠
应用 :
免疫印迹, 免疫组化
应用w/稀释 :
WB: 1:500;IHC: 1:100-1:500
反应物种 :
人类
免疫原 :
Recombinant protein within human p16INK4a aa 1-156.
抗体亚型 :
IgG
靶蛋白 :
p16INK4a
靶标.UniProt数据库 :
P42771
靶标.同义词 :
Cyclin-dependent kinase inhibitor 2
Cyclin-dependent kinase 4 inhibitor A
CDK4I
Multiple tumor suppressor 1
MTS-1
p16-INK4a
p16-INK4
p16INK4A
Target function/background :
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
产品形式 :
液体
纯化 :
蛋白质G亲和纯化
浓度 :
2微克/微升
储存缓冲液 :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
储存空调 :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
分子量 :
16 kDa
供应商 :
Huabio
Volume :
100微升
价格 :
325
launch date :
2021Q1
更多信息或购买 :
公司信息
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!