产品简要
公司名称 :
HUABIO
产品类型 :
抗体
产品名称 :
Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)
目录 :
HA600032
规格 :
100微升
价格 :
A2E1
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
A2E1
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化-石蜡切片
更多信息或购买 :
HUABIO 产品网页
图像
图像 1 :
HUABIO HA600032 图像 1
Western blot analysis of Pan-Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600032, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat heart tissue lysate Lane 1: Mouse smooth muscle tissue lysate Lane 1: Human skeletal muscle tissue lysate
图像 2 :
HUABIO HA600032 图像 2
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue using anti-Pan-Actin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600032, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
图像 3 :
HUABIO HA600032 图像 3
Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using anti-Pan-Actin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600032, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
产品信息
Catalog# :
HA600032
产品名称 :
Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)
类别 :
Mouse monoclonal primary
共轭 :
未共轭
克隆性 :
单克隆
克隆编号 :
A2E1
宿主 :
小鼠
应用 :
免疫印迹, 免疫组化-P
应用w/稀释 :
WB:1:500-1:2000;免疫组化-P:1:100-1:500
反应物种 :
人类, 小鼠, 大鼠
免疫原 :
合成多肽对应于N末端人的阿尔法肌动蛋白(平滑肌, 骨骼肌, 心脏肌肉)
抗体亚型 :
IgG1
靶蛋白 :
Pan-Actin
靶标.UniProt数据库 :
P68133
靶标.同义词 :
Actin, aortic smooth muscle, Alpha-actin-2,Cell growth-inhibiting gene 46 protein,ctin, aortic smooth muscle, intermediate form, ACTA2,Actin, alpha cardiac muscle 1,Alpha-cardiac actin,Actin, alpha cardiac muscle 1, intermediate form,ACTC1,Alpha-actin-1,Actin, alpha skeletal muscle,Actin, alpha skeletal muscle, intermediate form,ACTC
Target function/background :
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTC1 encodes cardiac muscle alpha actin. This isoform differs from the alpha actin that is expressed in skeletal muscle, ACTA1. Alpha cardiac actin is the major protein of the thin filament in cardiac sarcomeres, which are responsible for muscle contraction and generation of force to support the pump function of the heart. ACTA2 (actin alpha 2) is an actin protein with several aliases including alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actins are a family of globular multi-functional proteins that form microfilaments. ACTA2 is one of 6 different actin isoforms and is involved in the contractile apparatus of smooth muscle. ACTA2 (as with all the actins) is extremely highly conserved and found in nearly all mammals. Actin, alpha skeletal muscle is a protein that in humans is encoded by the ACTA1 gene. Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
产品形式 :
液体
纯化 :
蛋白质A亲和纯化
浓度 :
2微克/微升
储存缓冲液 :
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
储存空调 :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
分子量 :
42kDa
供应商 :
Huabio
Volume :
100微升
价格 :
325
launch date :
2021Q1
更多信息或购买 :
HUABIO 产品网页
公司信息
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
公司总部: 美国
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!