产品简要
公司名称 :
HUABIO
产品类型 :
抗体
产品名称 :
胰岛素样生长因子1受体小鼠单克隆抗体[D5-G2](HA600033)
目录 :
HA600033
规格 :
100微升
价格 :
D5-G2
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
D5-G2
反应物种 :
人类
应用 :
免疫印迹, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
HUABIO HA600033 图像 1
Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
图像 2 :
HUABIO HA600033 图像 2
Flow cytometric analysis of IGF1R was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600033, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
产品信息
Catalog# :
HA600033
产品名称 :
胰岛素样生长因子1受体小鼠单克隆抗体[D5-G2](HA600033)
类别 :
Mouse monoclonal primary
共轭 :
未共轭
克隆性 :
单克隆
克隆编号 :
D5-G2
宿主 :
小鼠
应用 :
免疫印迹, FC
应用w/稀释 :
WB: 1:500;FC: 1:50-1:100
反应物种 :
人类
免疫原 :
Recombinant protein within human IGF1R aa 600-1000.
抗体亚型 :
IgG1
靶蛋白 :
胰岛素样生长因子1受体
靶标.UniProt数据库 :
P08069
靶标.同义词 :
胰岛素样生长因子1受体胰岛素样生长因子Ⅰ受体IGF-I受体CD221胰岛素样生长因子1受体甲链胰岛素样生长因子1受体乙链
Target function/background :
Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis.
产品形式 :
液体
纯化 :
蛋白质G亲和纯化
浓度 :
2微克/微升
储存缓冲液 :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
储存空调 :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
分子量 :
Predicted band size: 155 kDa
供应商 :
Huabio
Volume :
100微升
价格 :
325
launch date :
2021Q1
更多信息或购买 :
公司信息
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
公司总部: 美国
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!