产品简要
公司名称 :
HUABIO
产品类型 :
抗体
产品名称 :
ALKBH1重组兔单克隆抗体[JE54-26](HA720024)
目录 :
HA720024
规格 :
100微升
价格 :
JE54-26
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
JE54-26
反应物种 :
人类
应用 :
免疫印迹, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
HUABIO HA720024 图像 1
Western blot analysis of ALKBH1 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720024, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
图像 2 :
HUABIO HA720024 图像 2
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720024, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
图像 3 :
HUABIO HA720024 图像 3
Immunohistochemical analysis of paraffin-embedded Human colon tissue using anti-ALKBH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720024, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
产品信息
Catalog# :
HA720024
产品名称 :
ALKBH1重组兔单克隆抗体[JE54-26](HA720024)
类别 :
Recombinant Rabbit monoclonal primary
共轭 :
未共轭
克隆性 :
单克隆
克隆编号 :
JE54-26
宿主 :
应用 :
免疫印迹, 免疫组化-P
应用w/稀释 :
WB: 1:500-1:2, 000;免疫组化-P:1:50-1:200
反应物种 :
人类
免疫原 :
Synthetic peptide within C-terminal Human ALKBH1.
抗体亚型 :
IgG
靶蛋白 :
ALKBH1
靶标.UniProt数据库 :
Q13686
靶标.同义词 :
ALKBH1
Target function/background :
Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Mainly acts as a tRNA demethylase by removing N1-methyladenine from various tRNAs, with a preference for N1-methyladenine at position 58 present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N1-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation. In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine to form 5-formylcytosine (f5c) at this position. mt-tRNA(Met) containing the f5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. N6-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing . Demethylates mRNAs containing N3-methylcytidine modification. Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity. Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation.
产品形式 :
液体
纯化 :
蛋白质A亲和纯化
浓度 :
1微克/微升
储存缓冲液 :
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
储存空调 :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
分子量 :
44 kDa
供应商 :
Huabio
Volume :
100微升
价格 :
355
launch date :
2021Q1
更多信息或购买 :
公司信息
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
公司总部: 美国
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!