产品简要
公司名称 :
HUABIO
产品类型 :
抗体
产品名称 :
CPSF73重组兔单克隆抗体[JE59-11](HA720025)
目录 :
HA720025
规格 :
100微升
价格 :
JE59-11
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
JE59-11
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
Western blot analysis of CPSF73 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
图像 2 :
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
图像 3 :
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CPSF73 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
产品信息
Catalog# :
HA720025
产品名称 :
CPSF73重组兔单克隆抗体[JE59-11](HA720025)
类别 :
Recombinant Rabbit monoclonal primary
共轭 :
未共轭
克隆性 :
单克隆
克隆编号 :
JE59-11
宿主 :
兔
应用 :
免疫印迹, 免疫组化-P, 免疫细胞化学
应用w/稀释 :
WB: 1:500-1:1, 000;免疫组化-P:1:50-1:200;ICC:1:50-1:200
反应物种 :
人类, 小鼠, 大鼠
免疫原 :
Synthetic peptide within C-terminal Human CPSF73.
抗体亚型 :
IgG
靶蛋白 :
CPSF73
靶标.UniProt数据库 :
Q9UKF6
靶标.同义词 :
CPSF3
Target function/background :
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively.
产品形式 :
液体
纯化 :
蛋白质A亲和纯化
浓度 :
1微克/微升
储存缓冲液 :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
储存空调 :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
分子量 :
77 kDa
供应商 :
Huabio
Volume :
100微升
价格 :
355
launch date :
2021Q1
更多信息或购买 :
公司信息
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!