产品简要
公司名称 :
HUABIO
产品类型 :
抗体
产品名称 :
ERK2单克隆抗体(M1407-3)
目录 :
M1407-3
规格 :
100微升
价格 :
325美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5-D2
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
Western blot analysis of ERK2 on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
图像 2 :
ICC staining ERK2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ERK2 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.
图像 3 :
ICC staining ERK2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ERK2 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorcª 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.
产品信息
目录号 :
M1407-3
产品类型 :
Mouse monoclonal primary
产品名称 :
ERK2单克隆抗体(M1407-3)
靶蛋白 :
ERK2
uniprot# :
P28482
UniProt数据库网址 :
https://www.uniprot.org/uniprot/P28482
Synynoms :
Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2) MAPK1 ERK2 PRKM1 PRKM2
免疫原 :
This antibody is produced by immunizing mice with recombinant protein of ERK2.
宿主 :
小鼠
抗体亚型 :
IgG1
克隆性 :
单克隆
克隆编号 :
5-D2
共轭 :
未共轭
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-P, FC
Reactivities :
人类, 小鼠, 大鼠
分子量 :
42 kDa
纯化 :
蛋白质A纯化
Positive Control :
A549, HepG2, NIH-3T3, human colon tissue, human prostate cancer tissue, Hela.
规格 :
100微升
美国价格 :
325美元
形式 :
液体
浓度 :
2微克/微升
储存缓冲液 :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
储存空调 :
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
更多信息或购买 :
公司信息
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!