Thermus aquaticus DNA Polymerase, Recombinant

产品简要

公司名称 :

Hytest

产品类型 :

蛋白

产品名称 :

Thermus aquaticus DNA Polymerase, Recombinant

7T1

更多信息或购买 :

Hytest 产品网页

文章摘录数: 2

参考文献
Kiviniemi M, Hermann R, Nurmi J, Ziegler A, Knip M, Simell O, et al. A high-throughput population screening system for the estimation of genetic risk for type 1 diabetes: an application for the TEDDY (the Environmental Determinants of Diabetes in the Young) study. Diabetes Technol Ther. 2007;9:460-72 pubmed
Kiviniemi M, Nurmi J, Lövgren T, Ilonen J. Locked nucleic acid (LNA) probes in high-throughput genetic analysis: application to an assay for type 1 diabetes-related HLA-DQB1 alleles. Clin Biochem. 2005;38:1015-22 pubmed

产品信息

产品名称 :

Thermus aquaticus DNA Polymerase, Recombinant

目录号 :

7T1

Spec:Description :

Taq DNA Polymerase (recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus strain YT-1) consists of a single polypeptide chain with a molecular weight 94 kDa. It is a highly processive 5'-3' DNA polymerase lacking 3'-5' exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. This Taq DNA polymerase has a nontemplate-dependent activity, which adds a single deoxyadenosine (A) to the 3' ends of PCR products (3'-A overhangs). This property allows easily and efficiently to ligate the PCR products in TA cloning vectors. Amplification of target DNA fragments from 100 bp to 4 Kb can be achieved with this enzyme

Categorization :

抗体

Spec:Specifications/Features :

Buffer: Storage and dilution buffer: 20 mM HEPES, pH 7.9, 125 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % Tween 20, 50 % glycerol. Reaction buffer 10x (Mg 2+ minus): 670 mM Tris-HCl, pH 8.8, 166 mM (NH4) 2 SO 4 0.1 % Tween 20. Other common PCR buffers for Taq-DNA polymerases can be used as well. Optimal concentration of MgCl2 is 1.0-2.5 mM.

Spec:Concentration :

5单位/l

Spec:Brand/Trademark :

HyTest Ltd

更多信息或购买 :

Hytest 产品网页

公司信息