GLO1单克隆抗体(Glo1a)
赛默飞世尔
目录: MA1-13029
小鼠 单克隆 (Glo1a)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
文章摘录数: 5

Western blot analysis of Glyoxylase (Glo1) was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a Goat anti-Mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077). For siRNA knock-down experiments HeLa and U20S cells were treated with 100nM Glo1 siRNA or control siRNA for 48 hours. Lysates were collected and Western blot analysis performed as described above.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human Prostate and Stomach cancer tissues. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:800 with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin. Images are displayed at 40X magnification. Results demonstrate cytoplasmic localization of Glyoxylase.
规格: 100微克
价格: 美国470.00
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GLO1单克隆抗体(Glo1a)
赛默飞世尔
目录: MA1-13029X
小鼠 单克隆 (Glo1a)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
文章摘录数: 5

Western blot analysis of Glyoxylase (Glo1) was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a Goat anti-Mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Pico (Product #34077). For siRNA knock-down experiments HeLa and U20S cells were treated with 100nM Glo1 siRNA or control siRNA for 48 hours. Lysates were collected and Western blot analysis performed as described above.
规格: 20微升
价格: 美国169.00
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GLO1多克隆抗体
赛默飞世尔
目录: PA5-77987
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国500.00
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GLO1多克隆抗体
赛默飞世尔
目录: PA5-79315
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微克
价格: 美国416.00
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GLO1重组兔单克隆抗体(043)
赛默飞世尔
目录: MA5-31148
domestic rabbit 重组 (043)
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微升
价格: 美国416.00
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GLO1单克隆抗体(GT552)
赛默飞世尔
目录: MA5-31469
小鼠 单克隆 (GT552)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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GLO1多克隆抗体
赛默飞世尔
目录: PA5-81397
domestic rabbit 多克隆
反应物种: 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫沉淀
规格: 100微升
价格: 美国305.00
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GLO1多克隆抗体
赛默飞世尔
目录: PA5-95997
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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GLO1重组兔单克隆抗体(ARC0969)
赛默飞世尔
目录: MA5-35481
domestic rabbit 重组 (ARC0969)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
规格: 100微升
价格: 美国446.00
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GLO1多克隆抗体
赛默飞世尔
目录: PA5-115287
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 美国446.00
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