产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Nanog Monoclonal Antibody (eBioMLC-51), eBioscience
目录 :
14-5761-80
规格 :
25微克
价格 :
美国159
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
eBioMLC-51
反应物种 :
小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 65
出版应用/物种/样本/稀释参考文献
  • 免疫组化-冰冻切片; 小鼠; 1:500; 图 3a
Xu P, Borges R, Fillatre J, de Oliveira Melo M, Cheng T, Thisse B, et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nat Commun. 2021;12:3277 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:1000; 图 1d
Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H. Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells. Development. 2018;145: pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200; 图 3f
Li M, Amaral P, Cheung P, Bergmann J, Kinoshita M, Kalkan T, et al. A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. elife. 2017;6: pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:800; 图 3e
Jang S, Choubey S, Furchtgott L, Zou L, Doyle A, Menon V, et al. Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states. elife. 2017;6: pubmed 出版商
  • 免疫细胞化学; 小鼠; 2500 ng/ml; 图 3
Illingworth R, Hölzenspies J, Roske F, Bickmore W, Brickman J. Polycomb enables primitive endoderm lineage priming in embryonic stem cells. elife. 2016;5: pubmed 出版商
  • 免疫组化; 小鼠; 1:200; 图 1b
Thamodaran V, Bruce A. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development. Open Biol. 2016;6: pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200; 图 2
  • 免疫组化; 小鼠; 1:200; 图 3
Martin Gonzalez J, Morgani S, Bone R, Bonderup K, Abelchian S, Brakebusch C, et al. Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports. 2016;7:177-91 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500; 图 3
Murakami K, Günesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed 出版商
  • 免疫组化; 小鼠; 1:100; 图 1
Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, et al. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. 2016;143:24-34 pubmed 出版商
  • 免疫细胞化学; 小鼠; 图 3d
Higuchi Y, Nguyen C, Yasuda S, McMillan M, Hasegawa K, Kahn M. Specific Direct Small Molecule p300/?-Catenin Antagonists Maintain Stem Cell Potency. Curr Mol Pharmacol. 2016;9:272-279 pubmed
  • 免疫细胞化学; 小鼠; 图 s5a
Nakamura T, Yabuta Y, Okamoto I, Aramaki S, Yokobayashi S, Kurimoto K, et al. SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression. Nucleic Acids Res. 2015;43:e60 pubmed 出版商
  • 免疫组化; 小鼠; 1:500
Bangs F, Schrode N, Hadjantonakis A, Anderson K. Lineage specificity of primary cilia in the mouse embryo. Nat Cell Biol. 2015;17:113-22 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠
Geula S, Moshitch Moshkovitz S, Dominissini D, Mansour A, Kol N, Salmon Divon M, et al. Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science. 2015;347:1002-6 pubmed 出版商
  • 免疫细胞化学; 小鼠
Gouti M, Tsakiridis A, Wymeersch F, Huang Y, Kleinjung J, Wilson V, et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol. 2014;12:e1001937 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:200
Doughton G, Wei J, Tapon N, Welham M, Chalmers A. Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling. PLoS ONE. 2014;9:e95434 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:500; 图 7
  • 免疫组化; 小鼠; 1:500; 图 4
Betschinger J, Nichols J, Dietmann S, Corrin P, Paddison P, Smith A. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell. 2013;153:335-47 pubmed 出版商
Kinoshita M, Barber M, Mansfield W, Cui Y, Spindlow D, Stirparo G, et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell. 2021;28:453-471.e8 pubmed 出版商
Bergert M, Lembo S, Sharma S, Russo L, Milovanović D, Gretarsson K, et al. Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation. Cell Stem Cell. 2021;28:209-216.e4 pubmed 出版商
Yamamoto M, Suwa Y, Sugiyama K, Okashita N, Kawaguchi M, Tani N, et al. PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during transition from primed to naïve pluripotency. J Cell Sci. 2020;: pubmed 出版商
Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, et al. Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. Sci Adv. 2020;6:eaaz6699 pubmed 出版商
Mulas C, Hodgson A, Kohler T, Agley C, Humphreys P, Kleine Brüggeney H, et al. Microfluidic platform for 3D cell culture with live imaging and clone retrieval. Lab Chip. 2020;20:2580-2591 pubmed 出版商
Rao C, Malaguti M, Mason J, Lowell S. The transcription factor E2A drives neural differentiation in pluripotent cells. Development. 2020;147: pubmed 出版商
Fischer S, Corujo Simon E, Lilao Garzon J, Stelzer E, Muñoz Descalzo S. The transition from local to global patterns governs the differentiation of mouse blastocysts. PLoS ONE. 2020;15:e0233030 pubmed 出版商
Velychko S, Adachi K, Kim K, Hou Y, MacCarthy C, Wu G, et al. Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cell. 2019;25:737-753.e4 pubmed 出版商
Li R, Zhong C, Yu Y, Liu H, Sakurai M, Yu L, et al. Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures. Cell. 2019;179:687-702.e18 pubmed 出版商
Pantier R, Tatar T, Colby D, Chambers I. Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency marker. Life Sci Alliance. 2019;2: pubmed 出版商
Vanheer L, Song J, De Geest N, Janiszewski A, Talon I, Provenzano C, et al. Tox4 modulates cell fate reprogramming. J Cell Sci. 2019;132: pubmed 出版商
Vardhana S, Arnold P, Rosen B, Chen Y, Carey B, Huangfu D, et al. Glutamine independence is a selectable feature of pluripotent stem cells. Nat Metab. 2019;1:676-687 pubmed 出版商
Stuart H, Stirparo G, Lohoff T, Bates L, Kinoshita M, Lim C, et al. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency. Cell Stem Cell. 2019;25:388-406.e8 pubmed 出版商
Lawrence M, Theunissen T, Lombard P, Adams D, Silva J. ZMYM2 inhibits NANOG-mediated reprogramming. Wellcome Open Res. 2019;4:88 pubmed 出版商
Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, et al. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Cell Stem Cell. 2018;23:266-275.e6 pubmed 出版商
Finley L, Vardhana S, Carey B, Alonso Curbelo D, Koche R, Chen Y, et al. Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Nat Cell Biol. 2018;20:565-574 pubmed 出版商
Cao K, Collings C, Morgan M, Marshall S, Rendleman E, Ozark P, et al. An Mll4/COMPASS-Lsd1 epigenetic axis governs enhancer function and pluripotency transition in embryonic stem cells. Sci Adv. 2018;4:eaap8747 pubmed 出版商
Yachie Kinoshita A, Onishi K, Ostblom J, Langley M, Pósfai E, Rossant J, et al. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions. Mol Syst Biol. 2018;14:e7952 pubmed 出版商
Corsinotti A, Wong F, Tatar T, Szczerbinska I, Halbritter F, Colby D, et al. Distinct SoxB1 networks are required for naïve and primed pluripotency. elife. 2017;6: pubmed 出版商
Ju Lee H, Bartsch D, Xiao C, Guerrero S, Ahuja G, Schindler C, et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun. 2017;8:1456 pubmed 出版商
Bessonnard S, Coqueran S, Vandormael Pournin S, Dufour A, Artus J, Cohen Tannoudji M. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation. Sci Rep. 2017;7:12285 pubmed 出版商
Nissen S, Perera M, Gonzalez J, Morgani S, Jensen M, Sneppen K, et al. Four simple rules that are sufficient to generate the mammalian blastocyst. PLoS Biol. 2017;15:e2000737 pubmed 出版商
Chronis C, Fiziev P, Papp B, Butz S, Bonora G, Sabri S, et al. Cooperative Binding of Transcription Factors Orchestrates Reprogramming. Cell. 2017;168:442-459.e20 pubmed 出版商
Jerabek S, Ng C, Wu G, Arauzo Bravo M, Kim K, Esch D, et al. Changing POU dimerization preferences converts Oct6 into a pluripotency inducer. EMBO Rep. 2017;18:319-333 pubmed 出版商
Schroter C, Rué P, Mackenzie J, Martinez Arias A. FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development. 2015;142:4205-16 pubmed 出版商
Economou C, Tsakiridis A, Wymeersch F, Gordon Keylock S, Dewhurst R, Fisher D, et al. Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation. BMC Dev Biol. 2015;15:35 pubmed 出版商
Morgani S, Brickman J. LIF supports primitive endoderm expansion during pre-implantation development. Development. 2015;142:3488-99 pubmed 出版商
Chantzoura E, Skylaki S, Menendez S, Kim S, Johnsson A, Linnarsson S, et al. Reprogramming Roadblocks Are System Dependent. Stem Cell Reports. 2015;5:350-64 pubmed 出版商
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Turner D, Rué P, Mackenzie J, Davies E, Martinez Arias A. Brachyury cooperates with Wnt/β-catenin signalling to elicit primitive-streak-like behaviour in differentiating mouse embryonic stem cells. BMC Biol. 2014;12:63 pubmed 出版商
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Le Bin G, Muñoz Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W, et al. Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development. 2014;141:1001-10 pubmed 出版商
Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, et al. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med. 2014;6:212-25 pubmed 出版商
Malaguti M, Nistor P, Blin G, Pegg A, Zhou X, Lowell S. Bone morphogenic protein signalling suppresses differentiation of pluripotent cells by maintaining expression of E-Cadherin. elife. 2013;2:e01197 pubmed 出版商
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Denholtz M, Bonora G, Chronis C, Splinter E, de Laat W, Ernst J, et al. Long-range chromatin contacts in embryonic stem cells reveal a role for pluripotency factors and polycomb proteins in genome organization. Cell Stem Cell. 2013;13:602-16 pubmed 出版商
Kumar R, Dimenna L, Schrode N, Liu T, Franck P, Muñoz Descalzo S, et al. AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes. Nature. 2013;500:89-92 pubmed 出版商
Morgani S, Canham M, Nichols J, Sharov A, Migueles R, Ko M, et al. Totipotent embryonic stem cells arise in ground-state culture conditions. Cell Rep. 2013;3:1945-57 pubmed 出版商
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Wang Y, Fan Y, Puga A. Dioxin exposure disrupts the differentiation of mouse embryonic stem cells into cardiomyocytes. Toxicol Sci. 2010;115:225-37 pubmed 出版商
Chen Y, Du Z, Yao Z. Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated counterparts. Cell Res. 2006;16:641-50 pubmed
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产品信息
产品类型 :
抗体
产品名称 :
Nanog Monoclonal Antibody (eBioMLC-51), eBioscience
目录# :
14-5761-80
规格 :
25微克
价格 :
美国159
克隆性 :
单克隆
纯度 :
亲和色谱
宿主 :
大鼠
反应物种 :
小鼠
应用 :
Immunocytochemistry: 2 µg/mL, Western Blot: 2 µg/mL
物种 :
小鼠
克隆 :
eBioMLC-51
抗体亚型 :
IgG2a, kappa
储存 :
4° C
描述 :
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 2 µg/mL, Western Blot: 2 µg/mL
别名 :
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国