产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eBioscience™
目录 :
14-9865-82
规格 :
100微克
价格 :
美国309.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
CR55T33
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 8
出版应用/物种/样本/稀释参考文献
  • 流式细胞仪; 人类; 1:100; 图 7b
Wang J, Chan B, Tong M, Paung Y, Jo U, MARTIN D, et al. Prolyl isomerization of FAAP20 catalyzed by PIN1 regulates the Fanconi anemia pathway. PLoS Genet. 2019;15:e1007983 pubmed 出版商
Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed 出版商
Schüler F, Weiss J, Lindner S, Lohmüller M, Herzog S, Spiegl S, et al. Checkpoint kinase 1 is essential for normal B cell development and lymphomagenesis. Nat Commun. 2017;8:1697 pubmed 出版商
Lang P, Nanjangud G, Sokolsky Papkov M, Shaw C, Hwang D, Parker J, et al. ATR maintains chromosomal integrity during postnatal cerebellar neurogenesis and is required for medulloblastoma formation. Development. 2016;143:4038-4052 pubmed
Lukas J, Lukas C, Bartek J. More than just a focus: The chromatin response to DNA damage and its role in genome integrity maintenance. Nat Cell Biol. 2011;13:1161-9 pubmed 出版商
Ward I, Chen J. Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress. J Biol Chem. 2001;276:47759-62 pubmed
Burma S, Chen B, Murphy M, Kurimasa A, Chen D. ATM phosphorylates histone H2AX in response to DNA double-strand breaks. J Biol Chem. 2001;276:42462-7 pubmed
Rogakou E, Pilch D, Orr A, Ivanova V, Bonner W. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem. 1998;273:5858-68 pubmed
产品信息
产品类型 :
抗体
产品名称 :
Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (CR55T33), eBioscience™
目录# :
14-9865-82
规格 :
100微克
价格 :
美国309.00
克隆性 :
单克隆
纯度 :
亲和色谱
宿主 :
小鼠
反应物种 :
人类, 小鼠
应用 :
Flow Cytometry: 0.03 µg/test, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: Assay-Dependent
物种 :
人类, 小鼠
克隆 :
CR55T33
抗体亚型 :
IgG1, kappa
储存 :
4° C
描述 :
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
格式 :
液体
应用w/稀释 :
Flow Cytometry: 0.03 µg/test, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: Assay-Dependent
别名 :
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国