domestic goat 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
Immunohistochemical staining of paraffin embedded of Human Prostate using PA5-18168 at a concentration of 5 ug/ml. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
Western Blot staining of HepG2 cell lysate using PA5-18168 at a concentration of 0.2 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
规格: 100微克
价格: 美国436.00
至产商
小鼠 单克隆 (3A8)
反应物种: 人类, 小鼠, 犬
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 犬
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
Western blot analysis of FOXA1 was performed by loading 25µg MDCK whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing phospho-MDCK (Product #MA1-091) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #) at a dilution of 1:10000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34080).
Immunofluorescent analysis of FOXA1 (green) in MDCK cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing FOXA1 (Product#MA1-091), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
规格: 100微克
价格: 美国420.00
至产商
domestic rabbit 重组 (JF10-02)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片