EGF单克隆抗体(4E11)
赛默飞世尔
目录: MA5-15606
小鼠 单克隆 (4E11)
反应物种: 人类, 小鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
文章摘录数: 2

Immunofluorescence analysis of NIH-3T3 cells using EGF antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
规格: 100微升
价格: 美国476.00
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EGF单克隆抗体(1H11)
赛默飞世尔
目录: M805
小鼠 单克隆 (1H11)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
文章摘录数: 1

Immunofluorescent analysis of EGF (green) in HeLa cells either left untreated (left panel) or treated with 10ng/ml EGF (right panel) for 5 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an EGF monoclonal antibody (Product # M805) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Human EGF was performed by loading 2 µg of recombinant human EGF (Lane 1) or supernatant from an EGF expression clone transfected in 293T cells onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with an EGF monoclonal antibody recognizing Human EGF (Product # M805) at a dilution of 1:5000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product # 34075).
规格: 200微克
价格: 美国420.00
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EGF重组兔单克隆抗体(3H11L6)
赛默飞世尔
目录: 701538
domestic rabbit 重组 (3H11L6)
反应物种: 人类
应用: 酶联免疫吸附测定, 免疫细胞化学
文章摘录数: 1
规格: 100微克
价格: 美国375.00
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EGF多克隆抗体
赛默飞世尔
目录: PA5-96195
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, dot blot
文章摘录数: 1
规格: 100微升
价格: 美国446.00
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EGF Polyclonal Antibody, PeproTech
赛默飞世尔
目录: 500-P45-100UG
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
文章摘录数: 1
规格: 100微克
价格: 美国270.00
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EGF Polyclonal Antibody, PeproTech
赛默飞世尔
目录: 500-P45-1MG
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
文章摘录数: 1
规格: 1毫克
价格: 美国1836.00
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EGF Polyclonal Antibody, PeproTech
赛默飞世尔
目录: 500-P45-50UG
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
文章摘录数: 1
规格: 50微克
价格: 美国211.00
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EGF单克隆抗体(S-177)
赛默飞世尔
目录: MA1-21367
小鼠 单克隆 (S-177)
反应物种: 人类
应用: 酶联免疫吸附测定
规格: 250微克
价格: 美国456.00
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EGF单克隆抗体(KT2)
赛默飞世尔
目录: MA1-72503
小鼠 单克隆 (KT2)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微克
价格: 美国389.00
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EGF单克隆抗体(9D7F11)
赛默飞世尔
目录: MA5-15571
小鼠 单克隆 (9D7F11)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化

Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung breast tissues using EGF antibody

Immunohistochemical analysis of paraffin-embedded human Uterus tissues using anFtEG antibody
规格: 100微升
价格: 美国460.00
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EGF单克隆抗体(3A8)
赛默飞世尔
目录: M806
小鼠 单克隆 (3A8)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

Immunofluorescent analysis of EGF (green) in HeLa cells either left untreated (left panel) or treated with 10ng/ml EGF (right panel) for 5 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an EGF monoclonal antibody (Product # M806) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Human EGF was performed by loading 2ug of recombinant human EGF (Lane 1) or supernatant from an EGF expression clone transfected in 293T cells onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with an EGF monoclonal antibody recognizing Human EGF (Product # M806) at a dilution of 1:5000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product # 34075).
规格: 200微克
价格: 美国400.00
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EGF单克隆抗体(3A8), 生物素
赛默飞世尔
目录: M806B
小鼠 单克隆 (3A8)
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格: 100微克
价格: 美国450.00
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EGF单克隆抗体(9A1)
赛默飞世尔
目录: M807
小鼠 单克隆 (9A1)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

Western blot analysis of Human EGF was performed by loading 2ug of recombinant human EGF (Lane 1) or supernatant from an EGF expression clone transfected in 293T cells onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with an EGF monoclonal antibody recognizing Human EGF (Product # M807) at a dilution of 1:5000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31430) at a dilution of 1:10,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product # 34075).

Immunofluorescent analysis of EGF (green) in HeLa cells either left serum starved (left panel) or treated with serum (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an EGF monoclonal antibody (Product # M807) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
规格: 200微克
价格: 美国400.00
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pro-EGF Polyclonal Antibody
赛默飞世尔
目录: PA5-29229
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片

Immunohistochemical analysis of paraffin-embedded Breast ca using EGF (PA5-29229) antibody at 1:250 dilution.

Western Blot analysis of pro-EGF using anti-pro-EGF Polyclonal Antibody (PA5-29229), in HCT116 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:2000.
规格: 100微升
价格: 美国436.00
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EGF重组多克隆抗体(3 HCLC)
赛默飞世尔
目录: 710569
domestic rabbit 单克隆 (3 HCLC)
反应物种: 人类
应用: 酶联免疫吸附测定
规格: 100微克
价格: 美国375.00
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EGF单克隆抗体(10827)
赛默飞世尔
目录: MA5-23704
小鼠 单克隆 (10827)
反应物种: 人类
应用: 酶联免疫吸附测定, 免疫组化
规格: 500微克
价格: 美国450.00
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EGF多克隆抗体
赛默飞世尔
目录: PA5-79185
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微克
价格: 美国385.00
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EGF多克隆抗体
赛默飞世尔
目录: PA5-85301
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国446.00
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EGF多克隆抗体
赛默飞世尔
目录: PA5-86588
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国436.00
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EGF多克隆抗体
赛默飞世尔
目录: PA5-87146
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国500.00
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EGF Polyclonal Antibody, Biotin, PeproTech
赛默飞世尔
目录: 500-P45BT-1MG
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格: 2 x 500微克
价格: 美国3672.00
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EGF Polyclonal Antibody, Biotin, PeproTech
赛默飞世尔
目录: 500-P45BT-25UG
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格: 25微克
价格: 美国211.00
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EGF Polyclonal Antibody, Biotin, PeproTech
赛默飞世尔
目录: 500-P45BT-50UG
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格: 50微克
价格: 美国270.00
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EGF重组兔单克隆抗体(JA14-15)
赛默飞世尔
目录: MA5-42471
domestic rabbit 重组 (JA14-15)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 免疫组化-石蜡切片
规格: 100微升
价格: 美国440.00
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EGF单克隆抗体(KT57)
赛默飞世尔
目录: MA5-49584
小鼠 单克隆 (KT57)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微克
价格: 美国559.00
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EGF重组兔单克隆抗体(18B12)
赛默飞世尔
目录: MA5-50191
domestic rabbit 重组 (18B12)
反应物种: 人类
应用: 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 免疫组化-石蜡切片
规格: 100微升
价格: 美国389.00
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EGF多克隆抗体
赛默飞世尔
目录: PA5-120228
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国440.00
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pro-EGF Polyclonal Antibody
赛默飞世尔
目录: PA5-142961
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微克
价格: 美国426.00
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