赛默飞世尔 Hspb1 抗体

小鼠单克隆 (G3.1)
反应物种: 人类, 小鼠, 大鼠, 犬
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 35

Western blot analysis of Heat Shock Protein 27 (HSP27) was performed by loading 50µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP27 monoclonal antibody (Product # MA3-015) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
规格: 100微升
价格: 美国477.00
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磷酸化HSP27(Ser15)多克隆抗体
赛默飞世尔
目录: PA1-016

domestic rabbit 多克隆
反应物种: 人类, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunoprecipitation of Phospho Heat Shock Protein (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). HeLa cell lysate (50ug) was loaded as a positive control (left lane). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016) at a dilution of 1:500 overnight rotating at 4°C, washed in TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho-HSP27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-016), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
规格: 100微克
价格: 美国400.00
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HSP27多克隆抗体
赛默飞世尔
目录: PA1-017

domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
文章摘录数: 2

规格: 100微克
价格: 美国471.00
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磷酸化HSP27(Ser15)多克隆抗体
赛默飞世尔
目录: PA1-018

domestic rabbit 多克隆
反应物种: 人类, 大鼠
修饰: 磷酸化
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
文章摘录数: 1

Immunoprecipitation of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed on HeLa cells treated with 10uM Anisomysin for 30 minutes. Antigen-antibody complexes were formed by incubating 500ug of whole cell lysate with 3ug of p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:500 overnight rotating at 4°C, washed with TBST, and probed with Clean-Blot IP Detection Reagent (Product # 21230) at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunofluorescent analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 (green) in HeLa cells either left untreated (left panel) or treated with 10uM Anisomysin (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Phospho Heat Shock Protein 27 (p-HSP27) pSer15 was performed by loading 50ug of HeLa cell lysates from untreated cells (left lane) or cells treated with 10uM Anisomycin (right lane) for 30 minutes and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a p-HSP27 pSer15 polyclonal antibody (Product # PA1-018) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed with TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
规格: 100微克
价格: 美国500.00
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HSP25多克隆抗体
赛默飞世尔
目录: PA1-25494

domestic rabbit 多克隆
反应物种: 豚鼠, 中国人仓鼠, 小鼠, 大鼠, pigs
应用: 免疫印迹, 免疫沉淀

规格: 50微升
价格: 美国496.00
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HSP27多克隆抗体
赛默飞世尔
目录: AHO1132

domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

规格: 100微克
价格: 美国480.00
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磷酸化HSP27(Ser78, Ser82)多克隆抗体
赛默飞世尔
目录: PA5-36912

domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化, 免疫组化-石蜡切片

规格: 100微升
价格: 美国446.00
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HSP27多克隆抗体
赛默飞世尔
目录: PA5-78010

domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

规格: 100微升
价格: 美国496.00
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磷酸化HSP27(Ser82)重组兔单克隆抗体(SN06-73)
赛默飞世尔
目录: MA5-32266

domestic rabbit 重组 (SN06-73)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

规格: 100微升
价格: 美国446.00
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HSP27重组兔单克隆抗体(JJ09-13)
赛默飞世尔
目录: MA5-32473

domestic rabbit 重组 (JJ09-13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

规格: 100微升
价格: 美国496.00
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磷酸化HSP27(Ser82)重组兔单克隆抗体(ARC1576)
赛默飞世尔
目录: MA5-35873

domestic rabbit 重组 (ARC1576)
反应物种: 人类, 大鼠
应用: 免疫印迹

规格: 100微升
价格: 美国460.00
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HSP27重组兔单克隆抗体(ARC0531)
赛默飞世尔
目录: MA5-35072

domestic rabbit 重组 (ARC0531)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学

规格: 100微升
价格: 美国436.00
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磷酸化HSP27(Ser82)单克隆抗体(GT357)
赛默飞世尔
目录: MA5-35924

小鼠单克隆 (GT357)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学

规格: 100微升
价格: 美国416.00
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HSP27单克隆抗体(10-8)
赛默飞世尔
目录: MA5-44616

小鼠单克隆 (10-8)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片

规格: 100微升
价格: 美国440.00
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HSP27单克隆抗体(8A7), APC
赛默飞世尔
目录: MA5-45121

小鼠单克隆 (8A7)
反应物种: 豚鼠, 中国人仓鼠, 人类, 小鼠, 大鼠, 犬, 牛, 家羊
共轭标签: APC
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

规格: 100微克
价格: 美国385.00
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HSP27单克隆抗体(8A7), 异硫氰酸荧光素
赛默飞世尔
目录: MA5-45122

小鼠单克隆 (8A7)
反应物种: 中国人仓鼠, 豚鼠, 人类, 小鼠, 大鼠, 犬, 牛, 家羊
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

规格: 100微克
价格: 美国385.00
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HSP27单克隆抗体(8A7), PerCP
赛默飞世尔
目录: MA5-45123

小鼠单克隆 (8A7)
反应物种: 中国人仓鼠, 豚鼠, 人类, 小鼠, 大鼠, 犬, 牛, 家羊
共轭标签: PerCP
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

规格: 100微克
价格: 美国385.00
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HSP27多克隆抗体
赛默飞世尔
目录: PA5-143536

鸡多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫细胞化学

规格: 500微升
价格: 美国1556.00
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HSP27多克隆抗体
赛默飞世尔
目录: PA5-143570

鸡多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫细胞化学

规格: 100微升
价格: 美国450.00
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