产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
鼠双微基因2单克隆抗体(IF2)
目录 :
33-7100
规格 :
50微克
价格 :
美国348.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
IF2
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 37
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 5a
Ren Z, Aerts J, Vandenplas H, Wang J, Gorbenko O, Chen J, et al. Phosphorylated STAT5 regulates p53 expression via BRCA1/BARD1-NPM1 and MDM2. Cell Death Dis. 2016;7:e2560 pubmed 出版商
  • 免疫印迹; 人类; 1:500; 图 2b
Wei T, Biskup E, Gjerdrum L, Niazi O, Ødum N, Gniadecki R. Ubiquitin-specific protease 2 decreases p53-dependent apoptosis in cutaneous T-cell lymphoma. Oncotarget. 2016;7:48391-48400 pubmed 出版商
  • 免疫组化; 人类
Saâda Bouzid E, Burel Vandenbos F, Ranchère Vince D, Birtwisle Peyrottes I, Chetaille B, Bouvier C, et al. Prognostic value of HMGA2, CDK4, and JUN amplification in well-differentiated and dedifferentiated liposarcomas. Mod Pathol. 2015;28:1404-14 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:50
Weingertner N, Neuville A, Chibon F, Ray Coquard I, Marcellin L, Ghnassia J. Myxoid liposarcoma with heterologous components: dedifferentiation or metaplasia? A FISH-documented and CGH-documented case report. Appl Immunohistochem Mol Morphol. 2015;23:230-5 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:50
Righi A, Gambarotti M, Benini S, Gamberi G, Cocchi S, Picci P, et al. MDM2 and CDK4 expression in periosteal osteosarcoma. Hum Pathol. 2015;46:549-53 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100
Ortega P, Suster D, Falconieri G, Zambrano E, Moran C, Morrison C, et al. Liposarcomas of the posterior mediastinum: clinicopathologic study of 18 cases. Mod Pathol. 2015;28:721-31 pubmed 出版商
  • 免疫印迹; 人类; 图 5
Qiu H, Zhang L, Ren C, Zeng Z, Wu W, Luo H, et al. Targeting CDH17 suppresses tumor progression in gastric cancer by downregulating Wnt/β-catenin signaling. PLoS ONE. 2013;8:e56959 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100
Yakkioui Y, Temel Y, Creytens D, Jahanshahi A, Fleischeuer R, Santegoeds R, et al. A comparison of cell-cycle markers in skull base and sacral chordomas. World Neurosurg. 2014;82:e311-8 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100; 表 1.
Yoshida A, Ushiku T, Motoi T, Beppu Y, Fukayama M, Tsuda H, et al. MDM2 and CDK4 immunohistochemical coexpression in high-grade osteosarcoma: correlation with a dedifferentiated subtype. Am J Surg Pathol. 2012;36:423-31 pubmed 出版商
  • 免疫组化; 人类; 1:25; 图 6
Dujardin F, Binh M, Bouvier C, Gomez Brouchet A, Larousserie F, Muret A, et al. MDM2 and CDK4 immunohistochemistry is a valuable tool in the differential diagnosis of low-grade osteosarcomas and other primary fibro-osseous lesions of the bone. Mod Pathol. 2011;24:624-37 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100; 图 2
Yoshida A, Ushiku T, Motoi T, Shibata T, Fukayama M, Tsuda H. Well-differentiated liposarcoma with low-grade osteosarcomatous component: an underrecognized variant. Am J Surg Pathol. 2010;34:1361-6 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100; 图 4
Yoshida A, Ushiku T, Motoi T, Shibata T, Beppu Y, Fukayama M, et al. Immunohistochemical analysis of MDM2 and CDK4 distinguishes low-grade osteosarcoma from benign mimics. Mod Pathol. 2010;23:1279-88 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100
Horvai A, Devries S, Roy R, O Donnell R, Waldman F. Similarity in genetic alterations between paired well-differentiated and dedifferentiated components of dedifferentiated liposarcoma. Mod Pathol. 2009;22:1477-88 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:50; 图 1
Daugaard S, Christensen L, Høgdall E. Markers aiding the diagnosis of chondroid tumors: an immunohistochemical study including osteonectin, bcl-2, cox-2, actin, calponin, D2-40 (podoplanin), mdm-2, CD117 (c-kit), and YKL-40. APMIS. 2009;117:518-25 pubmed 出版商
  • 免疫组化; 人类; 图 2
Nahal A, Meterissian S. Lipoleiomyosarcoma of the rectosigmoid colon: a unique site for a rare variant of liposarcoma. Am J Clin Oncol. 2009;32:353-5 pubmed 出版商
  • 免疫组化; 人类; 图 2
Amiot A, Dokmak S, Sauvanet A, Vilgrain V, Bringuier P, Scoazec J, et al. Sporadic desmoid tumor. An exceptional cause of cystic pancreatic lesion. JOP. 2008;9:339-45 pubmed
  • 免疫组化-石蜡切片; 人类; 1:100
Horvai A, Schaefer J, Nakakura E, O Donnell R. Immunostaining for peroxisome proliferator gamma distinguishes dedifferentiated liposarcoma from other retroperitoneal sarcomas. Mod Pathol. 2008;21:517-24 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:100; 表 1
Bosco M, Allia E, Coindre J, Odasso C, Pagani A, Pacchioni D. alpha-fetoprotein expression in a dedifferentiated liposarcoma. Virchows Arch. 2006;448:517-20 pubmed
  • 免疫组化-石蜡切片; 人类; 1:200; 表 1
Mentzel T, Toennissen J, Rutten A, Schaller J. Palmar atypical lipomatous tumour with spindle cell features (well-differentiated spindle cell liposarcoma): a rare neoplasm arising in an unusual anatomical location. Virchows Arch. 2005;446:300-4 pubmed
  • 免疫组化-冰冻切片; 人类; 1:100; 图 3
Coindre J, Hostein I, Maire G, Derré J, Guillou L, Leroux A, et al. Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity. J Pathol. 2004;203:822-30 pubmed
  • 免疫组化-石蜡切片; 人类
Coindre J, Mariani O, Chibon F, Mairal A, De Saint Aubain Somerhausen N, Favre Guillevin E, et al. Most malignant fibrous histiocytomas developed in the retroperitoneum are dedifferentiated liposarcomas: a review of 25 cases initially diagnosed as malignant fibrous histiocytoma. Mod Pathol. 2003;16:256-62 pubmed
Sladky V, Knapp K, Soratroi C, Heppke J, Eichin F, Rocamora Reverte L, et al. E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration. Dev Cell. 2020;52:335-349.e7 pubmed 出版商
Pellegrino M, Rizza P, Donà A, Nigro A, Ricci E, Fiorillo M, et al. FoxO3a as a Positive Prognostic Marker and a Therapeutic Target in Tamoxifen-Resistant Breast Cancer. Cancers (Basel). 2019;11: pubmed 出版商
Kim Y, Kim M, Park H, Yu D, Jung K, Song K, et al. Co-expression of MDM2 and CDK4 in transformed human mesenchymal stem cells causes high-grade sarcoma with a dedifferentiated liposarcoma-like morphology. Lab Invest. 2019;: pubmed 出版商
Cornillie J, Wozniak A, Li H, Wang Y, Boeckx B, Gebreyohannes Y, et al. Establishment and Characterization of Histologically and Molecularly Stable Soft-tissue Sarcoma Xenograft Models for Biological Studies and Preclinical Drug Testing. Mol Cancer Ther. 2019;18:1168-1178 pubmed 出版商
Berthier S, Larrouquere L, Champelovier P, Col E, Lefebvre C, Cottet Rouselle C, et al. A New Patient-Derived Metastatic Glioblastoma Cell Line: Characterisation and Response to Sodium Selenite Anticancer Agent. Cancers (Basel). 2018;11: pubmed 出版商
Wylleman R, Debiec Rychter M, Sciot R. A rare case of atypical/anaplastic meningioma with MDM2 amplification. Rare Tumors. 2018;10:2036361318779511 pubmed 出版商
Yang S, Lee J, Guo J, Kuo S, Tien Y, Kuo T, et al. Association of MDM2 expression with shorter progression-free survival and overall survival in patients with advanced pancreatic cancer treated with gemcitabine-based chemotherapy. PLoS ONE. 2017;12:e0180628 pubmed 出版商
Wang X, Zhang S, Bao J, Liu F. Oridonin synergizes with Nutlin-3 in osteosarcoma cells by modulating the levels of multiple Bcl-2 family proteins. Tumour Biol. 2017;39:1010428317701638 pubmed 出版商
Gottlieb A, Althoff K, Grunewald L, Thor T, Odersky A, Schulte M, et al. RITA displays anti-tumor activity in medulloblastomas independent of TP53 status. Oncotarget. 2017;8:27882-27891 pubmed 出版商
Grünewald I, Trautmann M, Busch A, Bauer L, Huss S, Schweinshaupt P, et al. MDM2 and CDK4 amplifications are rare events in salivary duct carcinomas. Oncotarget. 2016;7:75261-75272 pubmed 出版商
Helbig D, Ihle M, Pütz K, Tantcheva Poor I, Mauch C, Büttner R, et al. Oncogene and therapeutic target analyses in atypical fibroxanthomas and pleomorphic dermal sarcomas. Oncotarget. 2016;7:21763-74 pubmed 出版商
Künkele A, De Preter K, Heukamp L, Thor T, Pajtler K, Hartmann W, et al. Pharmacological activation of the p53 pathway by nutlin-3 exerts anti-tumoral effects in medulloblastomas. Neuro Oncol. 2012;14:859-69 pubmed 出版商
Weaver J, Goldblum J, Turner S, Tubbs R, Wang W, Lazar A, et al. Detection of MDM2 gene amplification or protein expression distinguishes sclerosing mesenteritis and retroperitoneal fibrosis from inflammatory well-differentiated liposarcoma. Mod Pathol. 2009;22:66-70 pubmed 出版商
Binh M, Garau X, Guillou L, Aurias A, Coindre J. Reproducibility of MDM2 and CDK4 staining in soft tissue tumors. Am J Clin Pathol. 2006;125:693-7 pubmed
Khor L, DeSilvio M, Al Saleem T, Hammond M, Grignon D, Sause W, et al. MDM2 as a predictor of prostate carcinoma outcome: an analysis of Radiation Therapy Oncology Group Protocol 8610. Cancer. 2005;104:962-7 pubmed
Wang Y, Lin R, Tan Y, Chen J, Chen C, Wang Y. Wild-type p53 overexpression and its correlation with MDM2 and p14ARF alterations: an alternative pathway to non-small-cell lung cancer. J Clin Oncol. 2005;23:154-64 pubmed
产品信息
产品类型 :
抗体
产品名称 :
鼠双微基因2单克隆抗体(IF2)
目录# :
33-7100
规格 :
50微克
价格 :
美国348.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类
应用 :
Immunocytochemistry: 1:25-1:200, Immunohistochemistry: 1:25-1:200, Immunoprecipitation: 1-5 µg/mL, Western Blot: 0.5-2 µg/mL
物种 :
人类
克隆 :
IF2
抗体亚型 :
IgG2b, kappa
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.
免疫原 :
Synthetic peptide derived from the n-terminal region of human MDM2
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:25-1:200, Immunohistochemistry: 1:25-1:200, Immunoprecipitation: 1-5 µg/mL, Western Blot: 0.5-2 µg/mL
别名 :
1700007J15Rik; AA415488; ACTFS; double minute 2; double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; HGNC:6973; human homolog of; MDM2; Mdm-2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog; MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 E3 ubiquitin protein ligase; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; transformed mouse 3T3 cell double minute 2
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国