Pan Ras Monoclonal Antibody (Ras10)
赛默飞世尔
目录: MA1-012X
小鼠 单克隆 (RAS10)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
文章摘录数: 12

Immunofluorescent analysis of Ras in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Ras staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Western blot analysis of Ras was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico(Product #34077).
规格: 20微克
价格: 美国149.00
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Pan Ras Monoclonal Antibody (Ras10)
赛默飞世尔
目录: MA1-012
小鼠 单克隆 (RAS10)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
文章摘录数: 13
规格: 100微克
价格: 美国425.00
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K-Ras Monoclonal Antibody (9.13)
赛默飞世尔
目录: 415700
小鼠 单克隆 (9.13)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 4
规格: 100微克
价格: 美国375.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-44339
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹
文章摘录数: 1
规格: 100微升
价格: 美国375.00
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K-Ras Monoclonal Antibody (F132-62)
赛默飞世尔
目录: MA1-91046
小鼠 单克隆 (F132-62)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
规格: 50微克
价格: 美国399.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-70104
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国375.00
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Pan Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-78035
domestic rabbit 多克隆
反应物种: thirteen-lined ground squirrel, 人类, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国425.00
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domestic rabbit 重组 (11H35L14)
反应物种: 人类
应用: 免疫印迹
规格: 100微克
价格: 美国325.00
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domestic rabbit 重组 (SA39-05)
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 流式细胞仪
规格: 100微升
价格: 美国375.00
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domestic rabbit 重组 (JF10-11)
反应物种: 人类, 小鼠, 大鼠, 斑马鱼
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国375.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-81515
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 美国249.00
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Pan Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-85947
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
规格: 100微升
价格: 美国375.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-88269
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国375.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-111300
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫组化
规格: 100微升
价格: 美国399.00
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domestic rabbit 重组 (ARC1112)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
规格: 100微升
价格: 美国375.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-102380
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国375.00
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Pan Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-104464
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 100微升
价格: 美国375.00
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K-Ras Polyclonal Antibody
赛默飞世尔
目录: PA5-109892
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格: 100微升
价格: 美国375.00
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domestic rabbit 重组 (ARC2369)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学
规格: 100微升
价格: 美国399.00
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磷酸化K(Ser89)多克隆抗体
赛默飞世尔
目录: PA5-117257
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片
规格: 100微升
价格: 美国399.00
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