缺氧诱导因子1A单克隆抗体(H1alpha67)
赛默飞世尔
目录: MA1-16504

小鼠 单克隆 (H1alpha67)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, pigs , 猫, 家羊, 非洲爪蛙, domestic rabbit
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, EMSA, 免疫组化-石蜡切片, 免疫组化-冰冻切片, proximity ligation assay
文章摘录数: 65

Western Blot analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts.

Immunohistochemical staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
规格: 100微升
价格: 美国459.00
至产商

缺氧诱导因子1A单克隆抗体(H1alpha67)
赛默飞世尔
目录: MA1-16511

小鼠 单克隆 (H1alpha67)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, pigs , 家羊, domestic rabbit
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 52

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-16511.

Immunoprecipitation of HIF-1 alpha; Heavy and light chains are also detected.

Western Blot detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
规格: 100微升
价格: 美国450.00
至产商

缺氧诱导因子1A单克隆抗体(H1alpha67), 生物素
赛默飞世尔
目录: MA1-46457

小鼠 单克隆 (H1alpha67)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, pigs , 家羊, domestic rabbit
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 39

Immunoprecipitation of HIF-1alpha using MA1-46457; Heavy and light chains are also detected.

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-46457.

On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
规格: 100微升
价格: 美国449.00
至产商

缺氧诱导因子1A单克隆抗体(mgc3)
赛默飞世尔
目录: MA1-516

小鼠 单克隆 (mgc3)
反应物种: 人类, 小鼠, 大鼠, 牛, pigs
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, EMSA, 免疫组化-石蜡切片, 抑制或激活实验
文章摘录数: 37

Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in Hela Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in U251 Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing HIF-1 alpha (MA1-516) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
规格: 100微升
价格: 美国516.00
至产商

HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 650
赛默飞世尔
目录: MA5-16008

小鼠 单克隆 (H1alpha67)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, pigs , 猫, 家羊, 非洲爪蛙, domestic rabbit
共轭标签: DyLight系列染料650
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 35

规格: 100微升
价格: 美国455.00
至产商

HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 550
赛默飞世尔
目录: MA5-16010

小鼠 单克隆 (H1alpha67)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, pigs , 猫, 家羊, 非洲爪蛙, domestic rabbit
共轭标签: DyLight系列染料550
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 34

规格: 100微升
价格: 美国449.00
至产商

HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 488
赛默飞世尔
目录: MA5-16009

小鼠 单克隆 (H1alpha67)
反应物种: 人类, 小鼠, 大鼠, 犬, 牛, pigs , 猫, 家羊, 非洲爪蛙, domestic rabbit
共轭标签: DyLight488
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 35

规格: 100微升
价格: 美国449.00
至产商

缺氧诱导因子1A多克隆抗体
赛默飞世尔
目录: PA1-16601

domestic rabbit 多克隆
反应物种: domestic goat, 中国人仓鼠, 人类, 小鼠, 大鼠, 犬, pigs , domestic rabbit
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 27

Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human placenta villi tissue section using PA1-16601.

Detection of HIF-1 Alpha (green) in RCC4 cells using PA1-16601. Nuclei (Blue) were counterstained using Hoechst 33258.

Western blot analysis of HIF-1 alpha was performed by loading 50 ug of Cos7 cell lysates under hypoxic (Lane 1: CoCl2 treated) or untreated (Lane 2) conditions onto a gel and then transferring blotted to membrane. Blot was probed with a rabbit polyclonal antibody recognizing HIF-1 alpha (Product # PA1-16601) and chemiluminescent deterction performed using ECL.
规格: 100微升
价格: 美国459.00
至产商

缺氧诱导因子1A多克隆抗体
赛默飞世尔
目录: PA1-16627

domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠, 牛, pigs
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Western Blot detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using PA1-16627.
规格: 100微升
价格: 美国499.00
至产商

HIF1A Polyclonal Antibody, DyLight™ 650
赛默飞世尔
目录: PA5-22777

domestic rabbit 多克隆
反应物种: 中国人仓鼠, domestic goat, 人类, 小鼠, 大鼠, 犬, pigs , domestic rabbit
共轭标签: DyLight系列染料650
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

规格: 100微升
价格: 美国449.00
至产商

HIF1A Polyclonal Antibody, DyLight™ 488
赛默飞世尔
目录: PA5-22778

domestic rabbit 多克隆
反应物种: 中国人仓鼠, domestic goat, 人类, 小鼠, 大鼠, 犬, pigs , domestic rabbit
共轭标签: DyLight488
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

规格: 100微升
价格: 美国449.00
至产商

缺氧诱导因子1A多克隆抗体, HRP
赛默飞世尔
目录: PA5-22779

domestic rabbit 多克隆
反应物种: domestic goat, 中国人仓鼠, 人类, 小鼠, 大鼠, 犬, pigs , domestic rabbit
共轭标签: HRP
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片, 免疫组化-冰冻切片

规格: 100微升
价格: 美国446.00
至产商

HIF1A Polyclonal Antibody, DyLight™ 550
赛默飞世尔
目录: PA5-22780

domestic rabbit 多克隆
反应物种: domestic goat, 中国人仓鼠, 人类, 小鼠, 大鼠, 犬, pigs , domestic rabbit
共轭标签: DyLight系列染料550
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

规格: 100微升
价格: 美国445.00
至产商