产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
GSK3甲/乙单克隆抗体(21A)
目录 :
44-610
规格 :
100微克
价格 :
美国460.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
21A
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 48
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 图 7a
Woodall B, Woodall M, Luongo T, Grisanti L, Tilley D, Elrod J, et al. Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy. J Biol Chem. 2016;291:21913-21924 pubmed
  • 免疫印迹; 小鼠; 图 1d
Diez H, Benitez M, Fernandez S, Torres Aleman I, Garrido J, Wandosell F. Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation. Biochim Biophys Acta. 2016;1863:2574-2583 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 4
Manassero G, Guglielmotto M, Zamfir R, Borghi R, Colombo L, Salmona M, et al. Beta-amyloid 1-42 monomers, but not oligomers, produce PHF-like conformation of Tau protein. Aging Cell. 2016;15:914-23 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 2
Franco Villanueva A, Wandosell F, Antón I. Neuritic complexity of hippocampal neurons depends on WIP-mediated mTORC1 and Abl family kinases activities. Brain Behav. 2015;5:e00359 pubmed 出版商
  • 免疫印迹; 小鼠; 1:3000; 图 2
Cymerman I, Gozdz A, Urbanska M, Milek J, Dziembowska M, Jaworski J. Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3β. PLoS ONE. 2015;10:e0134018 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Monteiro da Rocha A, Ding J, Slawny N, Wolf A, Smith G. Loss of glycogen synthase kinase 3 isoforms during murine oocyte growth induces offspring cardiac dysfunction. Biol Reprod. 2015;92:127 pubmed 出版商
  • 免疫印迹; 小鼠; 1:5000; 图 1
Ko R, Park J, Ha H, Choi Y, Lee S. Glycogen synthase kinase 3β ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine production. Nat Commun. 2015;6:6765 pubmed 出版商
  • 免疫印迹; 大鼠; 图 4d
  • 免疫印迹; 人类; 图 4a
Cuesto G, Jordán Álvarez S, Enriquez Barreto L, Ferrús A, Morales M, Acebes A. GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons. PLoS ONE. 2015;10:e0118475 pubmed 出版商
  • 免疫印迹; 人类; 图 s1
Banach Orlowska M, Szymańska E, Miaczynska M. APPL1 endocytic adaptor as a fine tuner of Dvl2-induced transcription. FEBS Lett. 2015;589:532-9 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Bhattacharjee R, Goswami S, Dudiki T, Popkie A, Phiel C, Kline D, et al. Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility. Biol Reprod. 2015;92:65 pubmed 出版商
  • 免疫印迹; 大鼠; 1:1000
Pérez Alvarez M, Mateos L, Alonso A, Wandosell F. Estradiol and Progesterone Administration After pMCAO Stimulates the Neurological Recovery and Reduces the Detrimental Effect of Ischemia Mainly in Hippocampus. Mol Neurobiol. 2015;52:1690-1703 pubmed 出版商
  • 免疫印迹; 小鼠
Maurin H, Lechat B, Borghgraef P, Devijver H, Jaworski T, Van Leuven F. Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3?/?. Eur J Neurosci. 2014;40:2442-53 pubmed 出版商
  • 免疫印迹; 小鼠; 1:4000; 图 3, 5
Wadosky K, Rodriguez J, Hite R, Min J, Walton B, Willis M. Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling. Am J Physiol Endocrinol Metab. 2014;306:E723-39 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Griffeth R, Carretero J, Burks D. Insulin receptor substrate 2 is required for testicular development. PLoS ONE. 2013;8:e62103 pubmed 出版商
  • 免疫印迹; 小鼠; 图 2
Maurin H, Lechat B, Dewachter I, Ris L, Louis J, Borghgraef P, et al. Neurological characterization of mice deficient in GSK3? highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase. Mol Brain. 2013;6:27 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000
Willis M, Min J, Wang S, McDonough H, Lockyer P, Wadosky K, et al. Carboxyl terminus of Hsp70-interacting protein (CHIP) is required to modulate cardiac hypertrophy and attenuate autophagy during exercise. Cell Biochem Funct. 2013;31:724-35 pubmed 出版商
  • 免疫印迹; 人类; 图 5
Zhou X, Wang H, Burg M, Ferraris J. Inhibitory phosphorylation of GSK-3? by AKT, PKA, and PI3K contributes to high NaCl-induced activation of the transcription factor NFAT5 (TonEBP/OREBP). Am J Physiol Renal Physiol. 2013;304:F908-17 pubmed 出版商
  • 免疫印迹; 小鼠
Wang H, Ducommun S, Quan C, Xie B, Li M, Wasserman D, et al. AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J. 2013;449:479-89 pubmed 出版商
  • 免疫印迹; 大鼠; 1:1000; 图 5
Pérez Alvarez M, Maza M, Anton M, Ordoñez L, Wandosell F. Post-ischemic estradiol treatment reduced glial response and triggers distinct cortical and hippocampal signaling in a rat model of cerebral ischemia. J Neuroinflammation. 2012;9:157 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 s2
Wray J, Kalkan T, Gómez López S, Eckardt D, Cook A, Kemler R, et al. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat Cell Biol. 2011;13:838-45 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 1
Goñi Oliver P, Avila J, Hernandez F. Calpain regulates N-terminal interaction of GSK-3? with 14-3-3?, p53 and PKB but not with axin. Neurochem Int. 2011;59:97-100 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
García Martínez J, Wullschleger S, Preston G, Guichard S, Fleming S, Alessi D, et al. Effect of PI3K- and mTOR-specific inhibitors on spontaneous B-cell follicular lymphomas in PTEN/LKB1-deficient mice. Br J Cancer. 2011;104:1116-25 pubmed 出版商
  • 免疫印迹; 人类; 图 3
García Martínez J, Moran J, Clarke R, Gray A, Cosulich S, Chresta C, et al. Ku-0063794 is a specific inhibitor of the mammalian target of rapamycin (mTOR). Biochem J. 2009;421:29-42 pubmed 出版商
  • 免疫印迹; 人类
Cole A, Sutherland C. Measuring GSK3 expression and activity in cells. Methods Mol Biol. 2008;468:45-65 pubmed 出版商
  • 免疫印迹; 大鼠; 1:6000; 图 6
Sun H, Xu B, Sheveleva E, Chen Q. LY294002 inhibits glucocorticoid-induced COX-2 gene expression in cardiomyocytes through a phosphatidylinositol 3 kinase-independent mechanism. Toxicol Appl Pharmacol. 2008;232:25-32 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
Bouskila M, Hirshman M, Jensen J, Goodyear L, Sakamoto K. Insulin promotes glycogen synthesis in the absence of GSK3 phosphorylation in skeletal muscle. Am J Physiol Endocrinol Metab. 2008;294:E28-35 pubmed
  • 免疫印迹; 小鼠; 图 3
Mora A, Sakamoto K, McManus E, Alessi D. Role of the PDK1-PKB-GSK3 pathway in regulating glycogen synthase and glucose uptake in the heart. FEBS Lett. 2005;579:3632-8 pubmed
Cao Y, Li H, Zhao J, Sun Y, Jin X, Lv S, et al. Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics. Evid Based Complement Alternat Med. 2022;2022:2264394 pubmed 出版商
Kisseleff E, Vigouroux R, Hottin C, Lourdel S, Thomas L, Shah P, et al. Glycogen Synthase Kinase 3 Regulates the Genesis of Displaced Retinal Ganglion Cells3. Eneuro. 2021;8: pubmed 出版商
Kopietz F, Alshuweishi Y, Bijland S, Alghamdi F, Degerman E, Sakamoto K, et al. A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner. Biochem J. 2021;478:633-646 pubmed 出版商
Draffin J, Sánchez Castillo C, Fernández Rodrigo A, Sánchez Sáez X, Avila J, Wagner F, et al. GSK3α, not GSK3β, drives hippocampal NMDAR-dependent LTD via tau-mediated spine anchoring. EMBO J. 2021;40:e105513 pubmed 出版商
Guglielmotto M, Manassero G, Vasciaveo V, Venezia M, Tabaton M, Tamagno E. Estrogens Inhibit Amyloid-β-Mediated Paired Helical Filament-Like Conformation of Tau Through Antioxidant Activity and miRNA 218 Regulation in hTau Mice. J Alzheimers Dis. 2020;77:1339-1351 pubmed 出版商
Kopietz F, Rupar K, Berggreen C, S xe4 ll J, Vertommen D, Degerman E, et al. Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels. Am J Physiol Endocrinol Metab. 2020;319:E459-E471 pubmed 出版商
Herrera J, Ordóñez Gutiérrez L, Fabrias G, Casas J, Morales A, Hernández G, et al. Ovarian Hormone-Dependent Effects of Dietary Lipids on APP/PS1 Mouse Brain. Front Aging Neurosci. 2019;11:346 pubmed 出版商
Dey S, Eisa A, Kline D, Wagner F, Abeysirigunawardena S, Vijayaraghavan S. Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs. FASEB J. 2020;34:1247-1269 pubmed 出版商
Zhong W, Darmani N. The pivotal role of glycogen synthase kinase 3 (GSK-3) in vomiting evoked by specific emetogens in the least shrew (Cryptotis parva). Neurochem Int. 2020;132:104603 pubmed 出版商
Martín Segura A, Ahmed T, Casadomé Perales Á, Palomares Perez I, Palomer E, Kerstens A, et al. Age-associated cholesterol reduction triggers brain insulin resistance by facilitating ligand-independent receptor activation and pathway desensitization. Aging Cell. 2019;18:e12932 pubmed 出版商
Freitas M, Silva J, Brothag C, Regadas Correia B, Fardilha M, Vijayaraghavan S. Isoform-specific GSK3A activity is negatively correlated with human sperm motility. Mol Hum Reprod. 2019;25:171-183 pubmed 出版商
Urbanska M, Kazmierska Grebowska P, Kowalczyk T, Caban B, Nader K, Pijet B, et al. GSK3β activity alleviates epileptogenesis and limits GluA1 phosphorylation. EBioMedicine. 2019;39:377-387 pubmed 出版商
Gozdz A, Nikolaienko O, Urbanska M, Cymerman I, Sitkiewicz E, Blazejczyk M, et al. GSK3? and GSK3? Phosphorylate Arc and Regulate its Degradation. Front Mol Neurosci. 2017;10:192 pubmed 出版商
Irimia J, Meyer C, Segvich D, Surendran S, DePaoli Roach A, Morral N, et al. Lack of liver glycogen causes hepatic insulin resistance and steatosis in mice. J Biol Chem. 2017;292:10455-10464 pubmed 出版商
Wullschleger S, Wasserman D, Gray A, Sakamoto K, Alessi D. Role of TAPP1 and TAPP2 adaptor binding to PtdIns(3,4)P2 in regulating insulin sensitivity defined by knock-in analysis. Biochem J. 2011;434:265-74 pubmed 出版商
Pearce L, Alton G, Richter D, Kath J, Lingardo L, Chapman J, et al. Characterization of PF-4708671, a novel and highly specific inhibitor of p70 ribosomal S6 kinase (S6K1). Biochem J. 2010;431:245-55 pubmed 出版商
Asenjo A, González Armas J, Villanueva N. Phosphorylation of human respiratory syncytial virus P protein at serine 54 regulates viral uncoating. Virology. 2008;380:26-33 pubmed 出版商
Kelleher I, Garwood C, Hanger D, Anderton B, Noble W. Kinase activities increase during the development of tauopathy in htau mice. J Neurochem. 2007;103:2256-67 pubmed
Taurin S, Sandbo N, Qin Y, Browning D, Dulin N. Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase. J Biol Chem. 2006;281:9971-6 pubmed
Medunjanin S, Hermani A, De Servi B, Grisouard J, Rincke G, Mayer D. Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity. J Biol Chem. 2005;280:33006-14 pubmed
McManus E, Sakamoto K, Armit L, Ronaldson L, Shpiro N, Marquez R, et al. Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis. EMBO J. 2005;24:1571-83 pubmed
产品信息
产品类型 :
抗体
产品名称 :
GSK3甲/乙单克隆抗体(21A)
目录# :
44-610
规格 :
100微克
价格 :
美国460.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠, 非洲爪蛙
应用 :
ELISA: 1:1,000, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: 5-10 µg, Western Blot: 1:1,000
物种 :
人类, 小鼠, 大鼠, 非洲爪蛙
克隆 :
21A
抗体亚型 :
IgG2a
储存 :
4°C short term, -80°C long term
描述 :
Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.
免疫原 :
The antibody was produced in mouse against a recombinant Xenopus laevis protein
格式 :
液体
应用w/稀释 :
ELISA: 1:1,000, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:100, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: 5-10 µg, Western Blot: 1:1,000
别名 :
2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; FA; factor A; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK3; GSK-3; GSK-3 alpha; GSK-3 beta; Gsk3a; gsk-3-alpha; Gsk3b; GSK-3beta; GSK3beta isoform; serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国