产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化FAK(Tyr397)单克隆抗体(141-9)
目录 :
44-625G
规格 :
10 blots
价格 :
美国450.00
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
141-9
反应物种 :
人类, 小鼠, 大鼠, , fruit fly , 非洲爪蛙
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 58
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:1000; 图 4b
Dragoj M, Milosević Z, Bankovic J, Tanic N, Pesic M, Stankovic T. Targeting CXCR4 and FAK reverses doxorubicin resistance and suppresses invasion in non-small cell lung carcinoma. Cell Oncol (Dordr). 2017;40:47-62 pubmed 出版商
  • 免疫印迹; 人类; 图 5
Alekhina O, Marchese A. ?-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4. J Biol Chem. 2016;291:26083-26097 pubmed
  • 免疫印迹; 人类; 图 s2
Richter E, Harms M, Ventz K, Nölker R, Fraunholz M, Mostertz J, et al. Quantitative Proteomics Reveals the Dynamics of Protein Phosphorylation in Human Bronchial Epithelial Cells during Internalization, Phagosomal Escape, and Intracellular Replication of Staphylococcus aureus. J Proteome Res. 2016;15:4369-4386 pubmed
  • 免疫印迹; 人类; 1:1000; 图 2b
Zeng F, Xie Y, Liao L, Li L, Chen B, Xie J, et al. Biological characterization of three immortalized esophageal epithelial cell lines. Mol Med Rep. 2016;14:4802-4810 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:25; 图 1a
Meehan D, Delimont D, Dufek B, Zallocchi M, Phillips G, Gratton M, et al. Endothelin-1 mediated induction of extracellular matrix genes in strial marginal cells underlies strial pathology in Alport mice. Hear Res. 2016;341:100-108 pubmed 出版商
  • 免疫组化; 小鼠; 1:200; 图 2
Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed 出版商
  • 免疫印迹; 人类; 图 5
Mukherjee D, Lu H, Yu L, He C, Lahiri S, Li T, et al. Krüppel-like factor 8 activates the transcription of C-X-C cytokine receptor type 4 to promote breast cancer cell invasion, transendothelial migration and metastasis. Oncotarget. 2016;7:23552-68 pubmed 出版商
  • 免疫细胞化学; 人类; 图 2
  • 免疫印迹; 人类; 图 2
Budina Kolomets A, Webster M, Leu J, Jennis M, Krepler C, Guerrini A, et al. HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors. Cancer Res. 2016;76:2720-30 pubmed 出版商
  • 免疫组化; 鸡; 1:200; 图 s1
Long K, Moss L, Laursen L, Boulter L, ffrench Constant C. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun. 2016;7:10354 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200; 图 6
  • 免疫印迹; 人类; 1:500; 图 5
  • 免疫细胞化学; 小鼠; 1:200; 图 s5
Horton E, Humphries J, Stutchbury B, Jacquemet G, Ballestrem C, Barry S, et al. Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition. J Cell Biol. 2016;212:349-64 pubmed 出版商
  • 免疫印迹; 人类; 1:500; 图 5
  • 免疫印迹; 小鼠; 1:500; 图 5
Wu J, Chen Y, Kuo C, Wenshin Yu H, Chen Y, Chiou A, et al. Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration. Sci Rep. 2015;5:18476 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200; 图 s7
Horton E, Byron A, Askari J, Ng D, Millon Frémillon A, Robertson J, et al. Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly. Nat Cell Biol. 2015;17:1577-1587 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 4
Hoekstra E, Kodach L, Das A, Ruela de Sousa R, Ferreira C, Hardwick J, et al. Low molecular weight protein tyrosine phosphatase (LMWPTP) upregulation mediates malignant potential in colorectal cancer. Oncotarget. 2015;6:8300-12 pubmed
  • 免疫印迹; 大鼠; 1:1000
Xiao X, Cheng C, Mruk D. Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat. J Endocrinol. 2013;216:73-86 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
Tomar A, Lawson C, Ghassemian M, Schlaepfer D. Cortactin as a target for FAK in the regulation of focal adhesion dynamics. PLoS ONE. 2012;7:e44041 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100; 图 6
Cole C, Hansen S, Barath M, Rushton G, Gardiner J, Avizienyte E, et al. Synthetic heparan sulfate oligosaccharides inhibit endothelial cell functions essential for angiogenesis. PLoS ONE. 2010;5:e11644 pubmed 出版商
  • 免疫印迹; 人类
Huot M, Brown C, Lamarche Vane N, Richard S. An adaptor role for cytoplasmic Sam68 in modulating Src activity during cell polarization. Mol Cell Biol. 2009;29:1933-43 pubmed 出版商
  • 免疫组化; 人类; 图 2
Lopes M, Ribeiro G, Tornatore T, Clemente C, Teixeira V, Franchini K. Increased expression and phosphorylation of focal adhesion kinase correlates with dysfunction in the volume-overloaded human heart. Clin Sci (Lond). 2007;113:195-204 pubmed
Bertrand Chapel A, Caligaris C, Fenouil T, Savary C, Aires S, Martel S, et al. SMAD2/3 mediate oncogenic effects of TGF-β in the absence of SMAD4. Commun Biol. 2022;5:1068 pubmed 出版商
Murphy K, REED D, Vennin C, Conway J, Nobis M, Yin J, et al. Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin status. Sci Adv. 2021;7:eabh0363 pubmed 出版商
Mousson A, Legrand M, Steffan T, Vauchelles R, Carl P, Gies J, et al. Inhibiting FAK-Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells. Cancers (Basel). 2021;13: pubmed 出版商
Kilinc A, Han S, Barrett L, Anandasivam N, Nelson C. Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFβ1. Mol Biol Cell. 2021;32:402-412 pubmed 出版商
Jones M, Askari J, Humphries J, Humphries M. Cell adhesion is regulated by CDK1 during the cell cycle. J Cell Biol. 2018;217:3203-3218 pubmed 出版商
Lee H, Lee Y, Choi H, Seok J, Yoon B, Kim D, et al. SCARA5 plays a critical role in the commitment of mesenchymal stem cells to adipogenesis. Sci Rep. 2017;7:14833 pubmed 出版商
Rashid M, Belmont J, Carpenter D, Turner C, Olson E. Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation. Development. 2017;144:4002-4014 pubmed 出版商
Michael I, Orebrand M, Lima M, Pereira B, Volpert O, Quaggin S, et al. Angiopoietin-1 deficiency increases tumor metastasis in mice. BMC Cancer. 2017;17:539 pubmed 出版商
Atherton P, Lausecker F, Harrison A, Ballestrem C. Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity. J Cell Sci. 2017;130:2277-2291 pubmed 出版商
Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed 出版商
Oudin M, Jonas O, Kosciuk T, Broye L, Guido B, Wyckoff J, et al. Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression. Cancer Discov. 2016;6:516-31 pubmed 出版商
Li N, Mruk D, Wong C, Lee W, Han D, Cheng C. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J. 2015;29:3788-805 pubmed 出版商
Wang C, Xiang R, Zhang X, Chen Y. Doxycycline inhibits leukemic cell migration via inhibition of matrix metalloproteinases and phosphorylation of focal adhesion kinase. Mol Med Rep. 2015;12:3374-3380 pubmed 出版商
Grossman H, Chuderland D, Ninio Many L, Hasky N, Kaplan Kraicer R, Shalgi R. A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation. FASEB J. 2015;29:3206-16 pubmed 出版商
Li L, Jiang H, Xie Y, Liao L, Cao H, Xu X, et al. Macrolide analog F806 suppresses esophageal squamous cell carcinoma (ESCC) by blocking β1 integrin activation. Oncotarget. 2015;6:15940-52 pubmed
Tancioni I, Miller N, Uryu S, Lawson C, Jean C, Chen X, et al. FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth. Breast Cancer Res. 2015;17:47 pubmed 出版商
Richter E, Harms M, Ventz K, Gierok P, Chilukoti R, Hildebrandt J, et al. A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin. PLoS ONE. 2015;10:e0122089 pubmed 出版商
Petersen S, Luo R, Liebscher I, Giera S, Jeong S, Mogha A, et al. The adhesion GPCR GPR126 has distinct, domain-dependent functions in Schwann cell development mediated by interaction with laminin-211. Neuron. 2015;85:755-69 pubmed 出版商
Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed 出版商
Ninio Many L, Grossman H, Levi M, Zilber S, Tsarfaty I, Shomron N, et al. MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells. Oncoscience. 2014;1:250-261 pubmed
Fouquet B, Nikolic J, Larrous F, Bourhy H, Wirblich C, Lagaudrière Gesbert C, et al. Focal adhesion kinase is involved in rabies virus infection through its interaction with viral phosphoprotein P. J Virol. 2015;89:1640-51 pubmed 出版商
Yoon H, Dehart J, Murphy J, Lim S. Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights. J Histochem Cytochem. 2015;63:114-28 pubmed 出版商
Rubashkin M, Cassereau L, Bainer R, DuFort C, Yui Y, Ou G, et al. Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate. Cancer Res. 2014;74:4597-611 pubmed 出版商
Cheshenko N, Trepanier J, González P, Eugenin E, Jacobs W, Herold B. Herpes simplex virus type 2 glycoprotein H interacts with integrin ?v?3 to facilitate viral entry and calcium signaling in human genital tract epithelial cells. J Virol. 2014;88:10026-38 pubmed 出版商
Lu H, Hu L, Yu L, Wang X, Urvalek A, Li T, et al. KLF8 and FAK cooperatively enrich the active MMP14 on the cell surface required for the metastatic progression of breast cancer. Oncogene. 2014;33:2909-17 pubmed 出版商
Patel H, Zich J, Serrels B, Rickman C, Hardwick K, Frame M, et al. Kindlin-1 regulates mitotic spindle formation by interacting with integrins and Plk-1. Nat Commun. 2013;4:2056 pubmed 出版商
Graf R, Barbero S, Keller N, Chen L, Uryu S, Schlaepfer D, et al. Src-inducible association of CrkL with procaspase-8 promotes cell migration. Cell Adh Migr. 2013;7:362-9 pubmed 出版商
Ludwig K, Tse E, Wang J. Colon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors. BMC Cancer. 2013;13:221 pubmed 出版商
Ninio Many L, Grossman H, Shomron N, Chuderland D, Shalgi R. microRNA-125a-3p reduces cell proliferation and migration by targeting Fyn. J Cell Sci. 2013;126:2867-76 pubmed 出版商
Qian X, Mruk D, Wong E, Lie P, Cheng C. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology. 2013;154:1907-20 pubmed 出版商
Orlando C, Ster J, Gerber U, Fawcett J, Raineteau O. Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner. J Neurosci. 2012;32:18009-17, 18017a pubmed 出版商
Xiao X, Mruk D, Cheng C. c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution. Am J Physiol Endocrinol Metab. 2013;304:E145-59 pubmed 出版商
Lie P, Mruk D, Mok K, Su L, Lee W, Cheng C. Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat. Proc Natl Acad Sci U S A. 2012;109:12562-7 pubmed 出版商
Lim S, Miller N, Chen X, Tancioni I, Walsh C, Lawson C, et al. Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression. J Cell Biol. 2012;197:907-19 pubmed 出版商
Li H, Song H, Luo J, Liang J, Zhao S, Su R. Knockdown of glucose-regulated protein 78 decreases the invasion, metalloproteinase expression and ECM degradation in hepatocellular carcinoma cells. J Exp Clin Cancer Res. 2012;31:39 pubmed 出版商
Jeanes A, Wang P, Moreno Layseca P, Paul N, Cheung J, Tsang R, et al. Specific ?-containing integrins exert differential control on proliferation and two-dimensional collective cell migration in mammary epithelial cells. J Biol Chem. 2012;287:24103-12 pubmed 出版商
Lawson C, Lim S, Uryu S, Chen X, Calderwood D, Schlaepfer D. FAK promotes recruitment of talin to nascent adhesions to control cell motility. J Cell Biol. 2012;196:223-32 pubmed 出版商
Fujita M, Mitsuhashi H, Isogai S, Nakata T, Kawakami A, Nonaka I, et al. Filamin C plays an essential role in the maintenance of the structural integrity of cardiac and skeletal muscles, revealed by the medaka mutant zacro. Dev Biol. 2012;361:79-89 pubmed 出版商
Tomar A, Lim S, Lim Y, Schlaepfer D. A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells. J Cell Sci. 2009;122:1852-62 pubmed 出版商
Bernard Trifilo J, Lim S, Hou S, Schlaepfer D, Ilic D. Analyzing FAK and Pyk2 in early integrin signaling events. Curr Protoc Cell Biol. 2006;Chapter 14:Unit 14.7 pubmed 出版商
产品信息
产品类型 :
抗体
产品名称 :
磷酸化FAK(Tyr397)单克隆抗体(141-9)
目录# :
44-625G
规格 :
10 blots
价格 :
美国450.00
克隆性 :
单克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
鸡, 果蝇, 人类, 小鼠, 大鼠, 非洲爪蛙
应用 :
Immunocytochemistry: Assay-dependent, Western Blot: Assay-dependent
物种 :
鸡, 果蝇, 人类, 小鼠, 大鼠, 非洲爪蛙
克隆 :
141-9
抗体亚型 :
IgG
储存 :
-20°C
描述 :
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
免疫原 :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 397. The sequence is conserved in mouse, rat, chicken and frog.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: Assay-dependent, Western Blot: Assay-dependent
别名 :
CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国