产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Phospho-Tau (Ser199) Polyclonal Antibody
目录 :
44-734G
规格 :
10 blots
价格 :
美国309.00
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠, 大鼠,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, immunohistochemistry - free floating section, 免疫印迹基因敲除验证
更多信息或购买 :
文章摘录数: 21
出版应用/物种/样品/稀释参考文献
  • 免疫细胞化学; 人类; 图 1d
  • 免疫印迹; 人类; 1:1000; 图 s5b
Tseng J, Xie L, Song S, Xie Y, Allen L, Ajit D, et al. The Deacetylase HDAC6 Mediates Endogenous Neuritic Tau Pathology. Cell Rep. 2017;20:2169-2183 pubmed 出版商
  • 免疫印迹; 小鼠; 图 3a
Zimova I, Brezovakova V, Hromádka T, Weisová P, Cubinkova V, Valachova B, et al. Human Truncated Tau Induces Mature Neurofibrillary Pathology in a Mouse Model of Human Tauopathy. J Alzheimers Dis. 2016;54:831-43 pubmed 出版商
  • 免疫印迹; 小鼠; 图 8
Winston C, Noël A, Neustadtl A, Parsadanian M, Barton D, Chellappa D, et al. Dendritic Spine Loss and Chronic White Matter Inflammation in a Mouse Model of Highly Repetitive Head Trauma. Am J Pathol. 2016;186:552-67 pubmed 出版商
  • 免疫印迹; 大鼠
  • 免疫印迹; 狗; 1:1000; 图 7
  • 免疫印迹; 小鼠
  • 免疫印迹; 人类
Smolek T, Madari A, Farbáková J, Kandrac O, Jadhav S, Cente M, et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J Comp Neurol. 2016;524:874-95 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
Wang Y, Zhang Y, Hu W, Xie S, Gong C, Iqbal K, et al. Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation. Sci Rep. 2015;5:15709 pubmed 出版商
  • 免疫印迹基因敲除验证; 人类; 1:2000; 图 2d
Takeda S, Wegmann S, Cho H, DeVos S, Commins C, Roe A, et al. Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain. Nat Commun. 2015;6:8490 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 9
Porquet D, Andrés Benito P, Griñán Ferré C, Camins A, Ferrer I, Canudas A, et al. Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8). Age (Dordr). 2015;37:9747 pubmed 出版商
  • 免疫印迹; 人类
Wang Y, Yang R, Gu J, Yin X, Jin N, Xie S, et al. Cross talk between PI3K-AKT-GSK-3β and PP2A pathways determines tau hyperphosphorylation. Neurobiol Aging. 2015;36:188-200 pubmed 出版商
  • 免疫印迹; 小鼠
Pedr s I, Petrov D, Allgaier M, Sureda F, Barroso E, Beas Zarate C, et al. Early alterations in energy metabolism in the hippocampus of APPswe/PS1dE9 mouse model of Alzheimer's disease. Biochim Biophys Acta. 2014;1842:1556-66 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000; 表 1
Petry F, Pelletier J, Bretteville A, Morin F, Calon F, Hébert S, et al. Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions. PLoS ONE. 2014;9:e94251 pubmed 出版商
  • 免疫印迹; 小鼠
Maurin H, Lechat B, Borghgraef P, Devijver H, Jaworski T, Van Leuven F. Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3?/?. Eur J Neurosci. 2014;40:2442-53 pubmed 出版商
  • 免疫印迹; 小鼠; 图 10
Maurin H, Lechat B, Dewachter I, Ris L, Louis J, Borghgraef P, et al. Neurological characterization of mice deficient in GSK3? highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase. Mol Brain. 2013;6:27 pubmed 出版商
  • 免疫印迹; 小鼠; 1:500; 图 6
Oaks A, Frankfurt M, Finkelstein D, Sidhu A. Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function. PLoS ONE. 2013;8:e60378 pubmed 出版商
  • 免疫印迹; 小鼠; 图 2
Park S, Jung H, Kim Y, Park T, Kim C, Choi H, et al. Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity. J Neurochem. 2012;123:856-65 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
Congdon E, Wu J, Myeku N, Figueroa Y, Herman M, Marinec P, et al. Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo. Autophagy. 2012;8:609-22 pubmed 出版商
  • immunohistochemistry - free floating section; 大鼠; 1:200; 图 2
  • 免疫印迹; 大鼠; 1:1000; 图 2
Bolognin S, Blanchard J, Wang X, Basurto Islas G, Tung Y, Kohlbrenner E, et al. An experimental rat model of sporadic Alzheimer's disease and rescue of cognitive impairment with a neurotrophic peptide. Acta Neuropathol. 2012;123:133-51 pubmed 出版商
  • 免疫组化; 人类; 1:400; 图 8-13
Wai M, Liang Y, Shi C, Cho E, Kung H, Yew D. Co-localization of hyperphosphorylated tau and caspases in the brainstem of Alzheimer's disease patients. Biogerontology. 2009;10:457-69 pubmed 出版商
Tran H, Sanchez L, Brody D. Inhibition of JNK by a peptide inhibitor reduces traumatic brain injury-induced tauopathy in transgenic mice. J Neuropathol Exp Neurol. 2012;71:116-29 pubmed 出版商
Wang X, Blanchard J, Kohlbrenner E, Clement N, Linden R, Radu A, et al. The carboxy-terminal fragment of inhibitor-2 of protein phosphatase-2A induces Alzheimer disease pathology and cognitive impairment. FASEB J. 2010;24:4420-32 pubmed 出版商
Yu Y, Run X, Liang Z, Li Y, Liu F, Liu Y, et al. Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases. J Neurochem. 2009;108:1480-94 pubmed 出版商
Liu F, Iqbal K, Grundke Iqbal I, Rossie S, Gong C. Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease. J Biol Chem. 2005;280:1790-6 pubmed
产品信息
产品类型 :
抗体
产品名称 :
Phospho-Tau (Ser199) Polyclonal Antibody
目录# :
44-734G
规格 :
10 blots
价格 :
美国309.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用 :
Functional Assay: Assay Dependent, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: Assay Dependent
物种 :
人类, 小鼠, 大鼠
抗体亚型 :
IgG
储存 :
-20°C
描述 :
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
免疫原 :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 199. The sequence is conserved in mouse and rat.
格式 :
Liquid
应用w/稀释 :
Functional Assay: Assay Dependent, Immunocytochemistry: 1 µg/mL, Immunofluorescence: 1 µg/mL, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: Assay Dependent
别名 :
MAPT, Microtubule-associated protein tau, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国