产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Phospho-Tau (Ser262) Polyclonal Antibody
目录 :
44-750G
规格 :
10 blots
价格 :
美国309.00
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, immunohistochemistry - free floating section, 免疫印迹基因敲除验证
更多信息或购买 :
文章摘录数: 20
出版应用/物种/样品/稀释参考文献
  • 免疫细胞化学; 人类; 图 1d
  • 免疫印迹; 人类; 1:1000; 图 s5b
Tseng J, Xie L, Song S, Xie Y, Allen L, Ajit D, et al. The Deacetylase HDAC6 Mediates Endogenous Neuritic Tau Pathology. Cell Rep. 2017;20:2169-2183 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 1a
Trzeciakiewicz H, Tseng J, Wander C, Madden V, Tripathy A, Yuan C, et al. A Dual Pathogenic Mechanism Links Tau Acetylation to Sporadic Tauopathy. Sci Rep. 2017;7:44102 pubmed 出版商
  • 免疫印迹; 人类; 1:2500; 图 4a
Sun W, Lee S, Huang X, Liu S, Inayathullah M, Kim K, et al. Attenuation of synaptic toxicity and MARK4/PAR1-mediated Tau phosphorylation by methylene blue for Alzheimer's disease treatment. Sci Rep. 2016;6:34784 pubmed 出版商
  • 免疫印迹; 小鼠; 图 6a
Dinkins M, Enasko J, Hernandez C, Wang G, Kong J, Helwa I, et al. Neutral Sphingomyelinase-2 Deficiency Ameliorates Alzheimer's Disease Pathology and Improves Cognition in the 5XFAD Mouse. J Neurosci. 2016;36:8653-67 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 3
Manassero G, Guglielmotto M, Zamfir R, Borghi R, Colombo L, Salmona M, et al. Beta-amyloid 1-42 monomers, but not oligomers, produce PHF-like conformation of Tau protein. Aging Cell. 2016;15:914-23 pubmed 出版商
  • 免疫印迹; 大鼠
  • 免疫印迹; 小鼠
  • 免疫组化-石蜡切片; 狗; 1:1000
Smolek T, Madari A, Farbáková J, Kandrac O, Jadhav S, Cente M, et al. Tau hyperphosphorylation in synaptosomes and neuroinflammation are associated with canine cognitive impairment. J Comp Neurol. 2016;524:874-95 pubmed 出版商
  • 免疫印迹基因敲除验证; 人类; 1:2000; 图 2d
Takeda S, Wegmann S, Cho H, DeVos S, Commins C, Roe A, et al. Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain. Nat Commun. 2015;6:8490 pubmed 出版商
  • 免疫印迹; 小鼠
Hu X, Li X, Zhao M, Gottesdiener A, Luo W, Paul S. Tau pathogenesis is promoted by Aβ1-42 but not Aβ1-40. Mol Neurodegener. 2014;9:52 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000; 表 1
Petry F, Pelletier J, Bretteville A, Morin F, Calon F, Hébert S, et al. Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions. PLoS ONE. 2014;9:e94251 pubmed 出版商
  • 免疫印迹; 牛; 图 4
Yamazaki A, Nishizawa Y, Matsuura I, Hayashi F, Usukura J, Bondarenko V. Microtubule-associated protein tau in bovine retinal photoreceptor rod outer segments: comparison with brain tau. Biochim Biophys Acta. 2013;1832:1549-59 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 6
Oaks A, Frankfurt M, Finkelstein D, Sidhu A. Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function. PLoS ONE. 2013;8:e60378 pubmed 出版商
  • 免疫印迹; 小鼠; 图 2
Park S, Jung H, Kim Y, Park T, Kim C, Choi H, et al. Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity. J Neurochem. 2012;123:856-65 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
Congdon E, Wu J, Myeku N, Figueroa Y, Herman M, Marinec P, et al. Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo. Autophagy. 2012;8:609-22 pubmed 出版商
  • immunohistochemistry - free floating section; 大鼠; 1:200; 图 2
  • 免疫印迹; 大鼠; 1:1000; 图 2
Bolognin S, Blanchard J, Wang X, Basurto Islas G, Tung Y, Kohlbrenner E, et al. An experimental rat model of sporadic Alzheimer's disease and rescue of cognitive impairment with a neurotrophic peptide. Acta Neuropathol. 2012;123:133-51 pubmed 出版商
  • 免疫印迹; 人类; 1:500
Spatara M, Robinson A. Transgenic mouse and cell culture models demonstrate a lack of mechanistic connection between endoplasmic reticulum stress and tau dysfunction. J Neurosci Res. 2010;88:1951-61 pubmed 出版商
  • 免疫印迹; 人类; 图 3
Ojala J, Sutinen E, Salminen A, Pirttila T. Interleukin-18 increases expression of kinases involved in tau phosphorylation in SH-SY5Y neuroblastoma cells. J Neuroimmunol. 2008;205:86-93 pubmed 出版商
Yu Y, Run X, Liang Z, Li Y, Liu F, Liu Y, et al. Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases. J Neurochem. 2009;108:1480-94 pubmed 出版商
Kelleher I, Garwood C, Hanger D, Anderton B, Noble W. Kinase activities increase during the development of tauopathy in htau mice. J Neurochem. 2007;103:2256-67 pubmed
Lambourne S, Sellers L, Bush T, Choudhury S, Emson P, Suh Y, et al. Increased tau phosphorylation on mitogen-activated protein kinase consensus sites and cognitive decline in transgenic models for Alzheimer's disease and FTDP-17: evidence for distinct molecular processes underlying tau abnormalities. Mol Cell Biol. 2005;25:278-93 pubmed
Haase C, Stieler J, Arendt T, Holzer M. Pseudophosphorylation of tau protein alters its ability for self-aggregation. J Neurochem. 2004;88:1509-20 pubmed
产品信息
产品类型 :
抗体
产品名称 :
Phospho-Tau (Ser262) Polyclonal Antibody
目录# :
44-750G
规格 :
10 blots
价格 :
美国309.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用 :
Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500-1:2000
物种 :
人类, 小鼠, 大鼠
抗体亚型 :
IgG
储存 :
-20°C
描述 :
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclindependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
免疫原 :
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 262.
格式 :
Liquid
应用w/稀释 :
Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500-1:2000
别名 :
MAPT, Microtubule-associated protein tau, MTBT1, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国