ERK1/ERK2单克隆抗体(ERK-7D8)
赛默飞世尔
目录: 13-6200
小鼠 单克隆 (ERK-7D8)
反应物种: 人类, 小鼠, 大鼠, 犬, 非洲爪蛙
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
文章摘录数: 46
规格: 200微克
价格: 美国450.00
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磷酸化ERK1/ERK2(Thr185, Tyr187)重组兔单克隆抗体(15H10
赛默飞世尔
目录: 700012
domestic rabbit 重组 (15H10L7)
反应物种: 人类, 小鼠, 大鼠, 犬
修饰: 磷酸化
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
文章摘录数: 13
规格: 100微克
价格: 美国425.00
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ERK1/ERK2单克隆抗体(K.913.4)
赛默飞世尔
目录: MA5-15134
domestic rabbit 单克隆 (K.913.4)
反应物种: 中国人仓鼠, 人类, 小鼠, 大鼠, 犬, 牛, pigs , 斑马鱼, fruit fly , roundworm
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片
文章摘录数: 7
规格: 200微升
价格: 美国496.00
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ERK2单克隆抗体(6F8)
赛默飞世尔
目录: MA1-099
小鼠 单克隆 (6F8)
反应物种: 人类, 小鼠, 大鼠, 犬
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

Western blot analysis of MAPK1 was performed by loading 25µg of A431 and K562 whole cell lysate and 10µg of HEK293T overexpression lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing MAPK1 (Product #MA1-099) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #32430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).

Immunofluorescent analysis of MAPK1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing MAPK1 (Product# MA1-099), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
规格: 100微克
价格: 美国400.00
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ERK2单克隆抗体(C1)
赛默飞世尔
目录: MA5-50855
小鼠 单克隆 (C1)
反应物种: 豚鼠, domestic goat, 人类, 小鼠, 大鼠, 犬, 鸡, 牛, pigs , 家羊
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 200微升
价格: 美国429.00
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ERK2单克隆抗体(C1)
赛默飞世尔
目录: MA5-50856
小鼠 单克隆 (C1)
反应物种: domestic goat, 豚鼠, 人类, 小鼠, 大鼠, 犬, 鸡, 牛, pigs , 家羊
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
规格: 20微升
价格: 美国169.00
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