产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化FAK(Tyr576)重组兔单克隆抗体(2H74L24)
目录 :
700013
规格 :
100微克
价格 :
美国425.00
克隆性 :
重组
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
2H74L24
反应物种 :
人类, 小鼠, 斑马鱼
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 7
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:200; 图 8
Petridou N, Skourides P. A ligand-independent integrin β1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed 出版商
  • 免疫印迹; 人类; 图 2a,2c
Min H, Yun H, Lee J, Lee H, Cho J, Jang H, et al. Targeting the insulin-like growth factor receptor and Src signaling network for the treatment of non-small cell lung cancer. Mol Cancer. 2015;14:113 pubmed 出版商
  • 免疫印迹; 小鼠
McGinnis L, Pelech S, Kinsey W. Post-ovulatory aging of oocytes disrupts kinase signaling pathways and lysosome biogenesis. Mol Reprod Dev. 2014;81:928-45 pubmed 出版商
  • 免疫印迹; 斑马鱼; 1:500; 图 3
Petridou N, Stylianou P, Christodoulou N, Rhoads D, Guan J, Skourides P. Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos. PLoS ONE. 2012;7:e42577 pubmed 出版商
Brudvig J, Cain J, Schmidt Grimminger G, Stumpo D, Roux K, Blackshear P, et al. MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation. Mol Neurobiol. 2018;55:8388-8402 pubmed 出版商
Yoon H, Dehart J, Murphy J, Lim S. Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights. J Histochem Cytochem. 2015;63:114-28 pubmed 出版商
Petridou N, Skourides P. FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis. Nat Commun. 2014;5:5240 pubmed 出版商
产品信息
产品类型 :
抗体
产品名称 :
磷酸化FAK(Tyr576)重组兔单克隆抗体(2H74L24)
目录# :
700013
规格 :
100微克
价格 :
美国425.00
克隆性 :
重组单克隆
纯度 :
蛋白质A
宿主 :
兔
反应物种 :
人类
应用 :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:10-1:100
物种 :
人类
克隆 :
2H74L24
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
免疫原 :
A peptide corresponding to amino acids 573-582 of Q05397.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:10-1:100
别名 :
CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国